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- W2224078765 abstract "This chapter presents factors that are considered in developing stringently anaerobic techniques and in describing the procedure and rationale of roll-tube method. A roll-tube method was developed in which agar medium was distributed as a thin layer over the internal surface of test tubes charged with an anaerobic atmosphere for the isolation of obligately anaerobic bacteria of the rumen. In the roll-tube method, exposure of bacteria and culture medium to air is avoided by displacing the air in the culture vessel with an oxygen-free gas, such as carbon dioxide, hydrogen, nitrogen, or mixtures of these gases. Carbon dioxide is the gas of choice because it is heavier than air, relatively cheap, and valuable in buffering. Vessels are stoppered under conditions preventing access of air. The cultures require no special incubators and can be removed and examined with no anaerobic precautions if kept stoppered. If opened, anaerobiosis can be continuously maintained during necessary manipulations, and the culture again closed without exposure to oxygen." @default.
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- W2224078765 date "1969-01-01" @default.
- W2224078765 modified "2023-10-14" @default.
- W2224078765 title "Chapter IV A Roll Tube Method for Cultivation of Strict Anaerobes" @default.
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- W2224078765 doi "https://doi.org/10.1016/s0580-9517(08)70503-8" @default.
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