Matches in SemOpenAlex for { <https://semopenalex.org/work/W222503955> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W222503955 endingPage "88" @default.
- W222503955 startingPage "579" @default.
- W222503955 abstract "The fusion of mouse NS-1 myeloma cells with spleen cells from mice chronically infected with Toxoplasma gondii resulted in eight clones of hybridomas producing monospecific antibodies against membrane or cytoplasmic antigens of Toxoplasma tachyzoites. One of the antibodies to a cytoplasmic determinant was an IgM; the others directed to membrane or cytoplasmic antigens belonged to the IgG2 or IgG3 isotypes. Antibodies of clones 1E11 (IgG3), 2G11, and 3E6 (IgG2) directed to membrane antigens, bound complement and were reactive in the complement-dependent cytotoxicity assay of Sabin-Feldman. These IgG2 antibodies were strongly agglutinating to parasites, whereas the IgG3 was relatively weak. Another IgG2 antibody (5B6), possibly recognizing a shared antigen of membrane and cytoplasm, exhibited a low titre in the cytotoxicity assay as well as in the agglutination assay. Two other antibodies to membrane antigens (2B7 and 2F8) as well as an antibody to a cytoplasmic antigen (3G3) did not bind complement and did not cause agglutination. The pattern of parasite staining produced by monoclonal antibodies to membrane antigens in an IFA test was different from that of polyvalent antisera. A strictly localized or 'beaded' staining was observed, as well as a smooth, rim fluorescence. Toxoplasma tachyzoites were surface radio-iodinated and the solubilized membrane proteins were immunoprecipitated with monoclonal antibodies and analysed by two-dimensional polyacrylamide gel electrophoresis. Two independently arising monoclonal antibodies to membrane antigens (2G11 and 3E6) consistently precipitated both the solubilized 35,000 and 14,000 mol. wt proteins, while 1E11 precipitated the 27,000 mol. wt protein." @default.
- W222503955 created "2016-06-24" @default.
- W222503955 creator A5012534523 @default.
- W222503955 creator A5045200976 @default.
- W222503955 date "1980-08-01" @default.
- W222503955 modified "2023-10-18" @default.
- W222503955 title "Serological and immunochemical characterization of monoclonal antibodies to Toxoplasma gondii." @default.
- W222503955 cites W1524166821 @default.
- W222503955 cites W1588885776 @default.
- W222503955 cites W1639090197 @default.
- W222503955 cites W1967719811 @default.
- W222503955 cites W1977461031 @default.
- W222503955 cites W2016133866 @default.
- W222503955 cites W2018289835 @default.
- W222503955 cites W2030522563 @default.
- W222503955 cites W2047780915 @default.
- W222503955 cites W2062147146 @default.
- W222503955 cites W2064540741 @default.
- W222503955 cites W2083453910 @default.
- W222503955 cites W2093309976 @default.
- W222503955 cites W2100837269 @default.
- W222503955 cites W2154318575 @default.
- W222503955 cites W2161629160 @default.
- W222503955 cites W2178787756 @default.
- W222503955 cites W2368884405 @default.
- W222503955 cites W2413424042 @default.
- W222503955 cites W2425358686 @default.
- W222503955 cites W51791873 @default.
- W222503955 cites W97674068 @default.
- W222503955 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1458103" @default.
- W222503955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7000687" @default.
- W222503955 hasPublicationYear "1980" @default.
- W222503955 type Work @default.
- W222503955 sameAs 222503955 @default.
- W222503955 citedByCount "23" @default.
- W222503955 crossrefType "journal-article" @default.
- W222503955 hasAuthorship W222503955A5012534523 @default.
- W222503955 hasAuthorship W222503955A5045200976 @default.
- W222503955 hasConcept C143508840 @default.
- W222503955 hasConcept C147483822 @default.
- W222503955 hasConcept C153911025 @default.
- W222503955 hasConcept C159047783 @default.
- W222503955 hasConcept C159654299 @default.
- W222503955 hasConcept C195616568 @default.
- W222503955 hasConcept C203014093 @default.
- W222503955 hasConcept C2778128430 @default.
- W222503955 hasConcept C542903549 @default.
- W222503955 hasConcept C86803240 @default.
- W222503955 hasConcept C91523082 @default.
- W222503955 hasConceptScore W222503955C143508840 @default.
- W222503955 hasConceptScore W222503955C147483822 @default.
- W222503955 hasConceptScore W222503955C153911025 @default.
- W222503955 hasConceptScore W222503955C159047783 @default.
- W222503955 hasConceptScore W222503955C159654299 @default.
- W222503955 hasConceptScore W222503955C195616568 @default.
- W222503955 hasConceptScore W222503955C203014093 @default.
- W222503955 hasConceptScore W222503955C2778128430 @default.
- W222503955 hasConceptScore W222503955C542903549 @default.
- W222503955 hasConceptScore W222503955C86803240 @default.
- W222503955 hasConceptScore W222503955C91523082 @default.
- W222503955 hasIssue "4" @default.
- W222503955 hasLocation W2225039551 @default.
- W222503955 hasOpenAccess W222503955 @default.
- W222503955 hasPrimaryLocation W2225039551 @default.
- W222503955 hasRelatedWork W1929717975 @default.
- W222503955 hasRelatedWork W1970157419 @default.
- W222503955 hasRelatedWork W1973692620 @default.
- W222503955 hasRelatedWork W2013808251 @default.
- W222503955 hasRelatedWork W2037509311 @default.
- W222503955 hasRelatedWork W2045728173 @default.
- W222503955 hasRelatedWork W2097364204 @default.
- W222503955 hasRelatedWork W2146940419 @default.
- W222503955 hasRelatedWork W2153515931 @default.
- W222503955 hasRelatedWork W222503955 @default.
- W222503955 hasVolume "40" @default.
- W222503955 isParatext "false" @default.
- W222503955 isRetracted "false" @default.
- W222503955 magId "222503955" @default.
- W222503955 workType "article" @default.