FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2225450310> ?p ?o ?g. }

• W2225450310 endingPage "3738" @default.
• W2225450310 startingPage "3730" @default.
• W2225450310 abstract "Cholesterol is an evolutionarily highly optimized molecule particularly known for its ability to condense the phospholipids in cellular membranes. Until recently, the accompanying increase in the chain order of the surrounding phospholipids was attributed to the planar and rigid tetracyclic ring structure of cholesterol. However, detailed investigations of cholesterol's aliphatic side chain demonstrated that this side chain is responsible for approximately half of the condensation effect. Therefore, we investigated the structure and dynamics of the aliphatic side chain of cholesterol using (2)H solid-state nuclear magnetic resonance (NMR) spectroscopy and microsecond timescale all-atom molecular dynamics (MD) simulations in four different model membranes: POPC, DPPC, PSM, and POPC/PSM (1 : 1 mol/mol) and at three different temperatures: 5 °C, 37 °C, and 50 °C. A cholesterol variant, in which 11 hydrogens of the aliphatic side chain were exchanged for deuterium, was used and the respective (2)H NMR spectra confirmed the axially asymmetric rotational diffusion of cholesterol in DPPC and PSM. Furthermore, NMR spectra indicated that some hydrogens showed an unexpected magnetic inequivalency. This finding was confirmed by all-atom molecular dynamics simulations and detailed analysis revealed that the hydrogens of the methylene groups at C22, C23, and C24 are magnetically inequivalent. This inequivalency is caused by steric clashes of the aliphatic side chain with the ring structure of cholesterol as well as the branched C21 methyl group. These excluded volume effects result in reduced conformational flexibility of the aliphatic side chain of cholesterol and explain its high order (order parameter of 0.78 for chain motions) and large contribution to the condensation effect. Additionally, the motional pattern of the side chain becomes highly anisotropic such that it shows larger fluctuations perpendicular to the ring plane of cholesterol with a biaxiality of the distribution of 0.046. Overall, our results shed light on the mechanism how the aliphatic side chain is able to contribute about half of the condensation effect of cholesterol." @default.
• W2225450310 created "2016-06-24" @default.
• W2225450310 creator A5004530946 @default.
• W2225450310 creator A5036126800 @default.
• W2225450310 creator A5037870803 @default.
• W2225450310 creator A5076394204 @default.
• W2225450310 creator A5084068287 @default.
• W2225450310 date "2016-01-01" @default.
• W2225450310 modified "2023-09-26" @default.
• W2225450310 title "Structure and dynamics of the aliphatic cholesterol side chain in membranes as studied by ²H NMR spectroscopy and molecular dynamics simulation" @default.
• W2225450310 cites W1864346626 @default.
• W2225450310 cites W1870355358 @default.
• W2225450310 cites W1908528441 @default.
• W2225450310 cites W1966339391 @default.
• W2225450310 cites W1967603731 @default.
• W2225450310 cites W1969733907 @default.
• W2225450310 cites W1974285362 @default.
• W2225450310 cites W1977436685 @default.
• W2225450310 cites W1980478162 @default.
• W2225450310 cites W1987033415 @default.
• W2225450310 cites W1988201012 @default.
• W2225450310 cites W1990394752 @default.
• W2225450310 cites W1992899488 @default.
• W2225450310 cites W1993801027 @default.
• W2225450310 cites W1995093903 @default.
• W2225450310 cites W1996886086 @default.
• W2225450310 cites W1996989293 @default.
• W2225450310 cites W2002505077 @default.
• W2225450310 cites W2006912694 @default.
• W2225450310 cites W2011915654 @default.
• W2225450310 cites W2015514684 @default.
• W2225450310 cites W2024372419 @default.
• W2225450310 cites W2024533912 @default.
• W2225450310 cites W2035687084 @default.
• W2225450310 cites W2041323669 @default.
• W2225450310 cites W2041803307 @default.
• W2225450310 cites W2042284982 @default.
• W2225450310 cites W2043890963 @default.
• W2225450310 cites W2049209046 @default.
• W2225450310 cites W2054406591 @default.
• W2225450310 cites W2061776255 @default.
• W2225450310 cites W2066358752 @default.
• W2225450310 cites W2066414494 @default.
• W2225450310 cites W2072366248 @default.
• W2225450310 cites W2076156331 @default.
• W2225450310 cites W2079919058 @default.
• W2225450310 cites W2082241118 @default.
• W2225450310 cites W2082907536 @default.
• W2225450310 cites W2090473761 @default.
• W2225450310 cites W2092341721 @default.
• W2225450310 cites W2095873315 @default.
• W2225450310 cites W2098384796 @default.
• W2225450310 cites W2103636724 @default.
• W2225450310 cites W2105891411 @default.
• W2225450310 cites W2108364101 @default.
• W2225450310 cites W2111484177 @default.
• W2225450310 cites W2125872855 @default.
• W2225450310 cites W2134762386 @default.
• W2225450310 cites W2141367941 @default.
• W2225450310 cites W2144679378 @default.
• W2225450310 cites W2149628585 @default.
• W2225450310 cites W2150981663 @default.
• W2225450310 cites W2162907973 @default.
• W2225450310 cites W2168685857 @default.
• W2225450310 cites W2330260612 @default.
• W2225450310 cites W2334503806 @default.
• W2225450310 cites W82875452 @default.
• W2225450310 doi "https://doi.org/10.1039/c5cp05084g" @default.
• W2225450310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26762541" @default.
• W2225450310 hasPublicationYear "2016" @default.
• W2225450310 type Work @default.
• W2225450310 sameAs 2225450310 @default.
• W2225450310 citedByCount "17" @default.
• W2225450310 countsByYear W22254503102016 @default.
• W2225450310 countsByYear W22254503102017 @default.
• W2225450310 countsByYear W22254503102018 @default.
• W2225450310 countsByYear W22254503102019 @default.
• W2225450310 countsByYear W22254503102020 @default.
• W2225450310 countsByYear W22254503102021 @default.
• W2225450310 countsByYear W22254503102022 @default.
• W2225450310 countsByYear W22254503102023 @default.
• W2225450310 crossrefType "journal-article" @default.
• W2225450310 hasAuthorship W2225450310A5004530946 @default.
• W2225450310 hasAuthorship W2225450310A5036126800 @default.
• W2225450310 hasAuthorship W2225450310A5037870803 @default.
• W2225450310 hasAuthorship W2225450310A5076394204 @default.
• W2225450310 hasAuthorship W2225450310A5084068287 @default.
• W2225450310 hasBestOaLocation W22254503101 @default.
• W2225450310 hasConcept C121332964 @default.
• W2225450310 hasConcept C1276947 @default.
• W2225450310 hasConcept C147597530 @default.
• W2225450310 hasConcept C178790620 @default.
• W2225450310 hasConcept C185592680 @default.
• W2225450310 hasConcept C201194858 @default.
• W2225450310 hasConcept C204921945 @default.
• W2225450310 hasConcept C2777454769 @default.
• W2225450310 hasConcept C2778565081 @default.
• W2225450310 hasConcept C2780943371 @default.

• next