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- W2225778818 abstract "Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a eukaryotic expression plasmid, pCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 cell line and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV." @default.
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- W2225778818 date "2007-10-01" @default.
- W2225778818 modified "2023-09-23" @default.
- W2225778818 title "Cloning and expression of VP2 gene of Infectious bursal disease virus in eukaryotic cells" @default.
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