FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2226069855> ?p ?o ?g. }

• W2226069855 abstract "Adenoviral gene transfer is a valuable tool in molecular biology research. In order to be an efficient and safe vector, adenovirus structure and infection mechanism as well as molecular biology of the used transgene need to be well studied. The aim of this study was to evaluate the role of adenovirus as a gene transfer vector from several perspectives. Adenovirus uses receptor-mediated endocytosis in order to enter the target cell. The effect of Rab5 GTPase on adenovirus entry and gene transfer efficiency was examined first. Next, adenovirus was used as an investigatory tool in the cardiovascular research, focused on clarifying the role of adrenomedullin (AM) in heart and vascular remodeling. Finally, a model of adenoviral gene transfer into skin fibroblasts was used. The role of Rab5 GTPase in the adenovirus endocytosis was examined in HeLa cells using Cy3labeled adenovirus, and gene transfer efficiency using β-galactosidase encoding adenovirus. Rab5 increased both adenovirus uptake and gene transfer, whereas dominant negative Rab5S34N decreased both endocytosis and gene transfer. The data indicate that Rab5 is needed in mediating the adenovirus uptake into the target cell. In the rat heart, adenovirus-mediated AM gene transfer transiently improved systolic function both in vivo and in vitro. AM caused activation of translocation of protein kinases C e and δ, whereas phosphorylation of p38 mitogen activated protein kinase was decreased in the left ventricle. AM significantly attenuated the development of angiotensin II-induced cardiac hypertrophy. In rats with myocardial infarction, AM enhanced dilatation of left ventricle and thinning of anterior wall. The role of AM in neointima formation was evaluated in rat artery after endothelial injury. Intravascular AM gene transfer decreased neointimal growth and increased neointimal myofibroblasts apoptosis. These results show that AM regulates left ventricular systolic function and remodeling in the heart, and plays a role in pathological vascular remodeling. Adenovirus-mediated lysyl hydroxylase (LH) gene transfer into skin fibroblasts of type VI EhlersDanlos syndrome patient and rat skin increased functional LH production, elevated LH activity, and human LH mRNA production both in vitro and in vivo. LH gene replacement therapy may thus lead to possibilities to improve skin wound healing in Ehlers-Danlos syndrome patients." @default.
• W2226069855 created "2016-06-24" @default.
• W2226069855 creator A5089682623 @default.
• W2226069855 date "2004-01-01" @default.
• W2226069855 modified "2023-09-23" @default.
• W2226069855 title "Adenovirus endocytosis and adenoviral gene transfer in cardiovascular and dermatologic disease models" @default.
• W2226069855 cites W1486052360 @default.
• W2226069855 cites W1491410831 @default.
• W2226069855 cites W1492396077 @default.
• W2226069855 cites W1492654684 @default.
• W2226069855 cites W1507887565 @default.
• W2226069855 cites W1509922358 @default.
• W2226069855 cites W1517150667 @default.
• W2226069855 cites W1526496445 @default.
• W2226069855 cites W1539442102 @default.
• W2226069855 cites W1543445053 @default.
• W2226069855 cites W1563901359 @default.
• W2226069855 cites W1575957636 @default.
• W2226069855 cites W1582164157 @default.
• W2226069855 cites W1595083713 @default.
• W2226069855 cites W1597577233 @default.
• W2226069855 cites W1597663953 @default.
• W2226069855 cites W1600485881 @default.
• W2226069855 cites W1606338704 @default.
• W2226069855 cites W1606409542 @default.
• W2226069855 cites W1614056212 @default.
• W2226069855 cites W1630481045 @default.
• W2226069855 cites W169914313 @default.
• W2226069855 cites W1734478427 @default.
• W2226069855 cites W1753252347 @default.
• W2226069855 cites W1756200251 @default.
• W2226069855 cites W1798479690 @default.
• W2226069855 cites W1857650329 @default.
• W2226069855 cites W1877406376 @default.
• W2226069855 cites W1895535125 @default.
• W2226069855 cites W1902070349 @default.
• W2226069855 cites W1963705978 @default.
• W2226069855 cites W1964073548 @default.
• W2226069855 cites W1964441922 @default.
• W2226069855 cites W1964882417 @default.
• W2226069855 cites W1965260691 @default.
• W2226069855 cites W1966098523 @default.
• W2226069855 cites W1966182457 @default.
• W2226069855 cites W1966969839 @default.
• W2226069855 cites W1967934455 @default.
• W2226069855 cites W1968584991 @default.
• W2226069855 cites W1969008824 @default.
• W2226069855 cites W1969560454 @default.
• W2226069855 cites W1971629708 @default.
• W2226069855 cites W1973912404 @default.
• W2226069855 cites W1975667857 @default.
• W2226069855 cites W1976528399 @default.
• W2226069855 cites W1977303922 @default.
• W2226069855 cites W1977624384 @default.
• W2226069855 cites W1981492597 @default.
• W2226069855 cites W1983728153 @default.
• W2226069855 cites W1985317029 @default.
• W2226069855 cites W1985724556 @default.
• W2226069855 cites W1986068964 @default.
• W2226069855 cites W1986513627 @default.
• W2226069855 cites W1986579477 @default.
• W2226069855 cites W1986912541 @default.
• W2226069855 cites W1987154673 @default.
• W2226069855 cites W1987219070 @default.
• W2226069855 cites W1987968808 @default.
• W2226069855 cites W1987990767 @default.
• W2226069855 cites W1988169167 @default.
• W2226069855 cites W1988460287 @default.
• W2226069855 cites W1989669523 @default.
• W2226069855 cites W1989880720 @default.
• W2226069855 cites W1990187729 @default.
• W2226069855 cites W1990256292 @default.
• W2226069855 cites W1990561446 @default.
• W2226069855 cites W1993002467 @default.
• W2226069855 cites W1994734582 @default.
• W2226069855 cites W1996115257 @default.
• W2226069855 cites W1996141717 @default.
• W2226069855 cites W1997810397 @default.
• W2226069855 cites W1998149067 @default.
• W2226069855 cites W1998391508 @default.
• W2226069855 cites W1999673185 @default.
• W2226069855 cites W1999812791 @default.
• W2226069855 cites W2001758369 @default.
• W2226069855 cites W2002017720 @default.
• W2226069855 cites W2002331850 @default.
• W2226069855 cites W2003671607 @default.
• W2226069855 cites W2004512194 @default.
• W2226069855 cites W2005010203 @default.
• W2226069855 cites W2005062689 @default.
• W2226069855 cites W2005574904 @default.
• W2226069855 cites W2005760229 @default.
• W2226069855 cites W2006067898 @default.
• W2226069855 cites W2006809403 @default.
• W2226069855 cites W2007797957 @default.
• W2226069855 cites W2007903872 @default.
• W2226069855 cites W200862001 @default.
• W2226069855 cites W2009338295 @default.
• W2226069855 cites W2010488494 @default.
• W2226069855 cites W2011030446 @default.
• W2226069855 cites W2012978268 @default.

• next