FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2226089114> ?p ?o ?g. }

• W2226089114 endingPage "419" @default.
• W2226089114 startingPage "413" @default.
• W2226089114 abstract "We have investigated the intracellular distribution of apolipoprotein B (apo B) in rabbit liver by immunoblotting, radioimmunoassay (r.i.a.) and enzyme-linked immunoassay (e.l.i.s.a.). Apo B100 was detected in total microsomes, rough microsomes, smooth microsomes, trans-enriched Golgi and cis-enriched Golgi and membrane and cisternal-content subfractions prepared from these fractions. There was also evidence of degradation of apo B100 in the Golgi membrane fractions. The amount of apo B in the subcellular fractions detected by competitive r.i.a. or e.l.i.s.a. ranged from 1.5 micrograms/mg of protein in the rough endoplasmic reticulum to 13 micrograms/mg of protein in the trans-Golgi fraction. Using internal standards (NADPH-cytochrome c reductase for the endoplasmic reticulum and galactosyltransferase for the Golgi membranes) it was calculated that all the apo B of liver is recovered within the secretory compartment, with 63% of the total apo B in the endoplasmic reticulum and the remainder in the Golgi. When the subcellular fractions were separated into membranes and cisternal contents, 60%, 50%, 60% and 30% of the total apo B was recovered in the membrane of the rough microsomes, smooth microsomes, cis-Golgi and trans-Golgi respectively. Using competitive e.l.i.s.a. we found that the membrane-bound form of the apo B was exposed at the cytosolic surface of the intact subcellular fractions. These observations are consistent with a model for assembly of very-low-density lipoproteins (VLDL) in which newly synthesized apo B is incorporated into a membrane-bound pool and a lumenal pool. The membrane-bound pool not used for VLDL assembly may be degraded, possibly in the Golgi region." @default.
• W2226089114 created "2016-06-24" @default.
• W2226089114 creator A5009688263 @default.
• W2226089114 creator A5032973962 @default.
• W2226089114 creator A5048670504 @default.
• W2226089114 creator A5066217077 @default.
• W2226089114 creator A5074903646 @default.
• W2226089114 date "1992-12-01" @default.
• W2226089114 modified "2023-09-23" @default.
• W2226089114 title "Determination of the intracellular distribution and pool sizes of apolipoprotein B in rabbit liver" @default.
• W2226089114 cites W1489766749 @default.
• W2226089114 cites W1492377313 @default.
• W2226089114 cites W1498346306 @default.
• W2226089114 cites W1518225155 @default.
• W2226089114 cites W1522459970 @default.
• W2226089114 cites W1599366995 @default.
• W2226089114 cites W1775749144 @default.
• W2226089114 cites W1921601722 @default.
• W2226089114 cites W1935546453 @default.
• W2226089114 cites W1940347886 @default.
• W2226089114 cites W1956183677 @default.
• W2226089114 cites W1983170203 @default.
• W2226089114 cites W2004270134 @default.
• W2226089114 cites W2024879590 @default.
• W2226089114 cites W2027270726 @default.
• W2226089114 cites W2031423487 @default.
• W2226089114 cites W2032731208 @default.
• W2226089114 cites W2037398430 @default.
• W2226089114 cites W2047600123 @default.
• W2226089114 cites W2063739195 @default.
• W2226089114 cites W2078486939 @default.
• W2226089114 cites W2135503869 @default.
• W2226089114 cites W2137425178 @default.
• W2226089114 cites W2140269404 @default.
• W2226089114 cites W2147962799 @default.
• W2226089114 cites W2157680057 @default.
• W2226089114 cites W2160107586 @default.
• W2226089114 cites W2164162223 @default.
• W2226089114 cites W2338060946 @default.
• W2226089114 cites W2339557541 @default.
• W2226089114 cites W2394146660 @default.
• W2226089114 cites W2402354430 @default.
• W2226089114 cites W2408363620 @default.
• W2226089114 cites W2416396728 @default.
• W2226089114 doi "https://doi.org/10.1042/bj2880413" @default.
• W2226089114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1132026" @default.
• W2226089114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1463445" @default.
• W2226089114 hasPublicationYear "1992" @default.
• W2226089114 type Work @default.
• W2226089114 sameAs 2226089114 @default.
• W2226089114 citedByCount "23" @default.
• W2226089114 crossrefType "journal-article" @default.
• W2226089114 hasAuthorship W2226089114A5009688263 @default.
• W2226089114 hasAuthorship W2226089114A5032973962 @default.
• W2226089114 hasAuthorship W2226089114A5048670504 @default.
• W2226089114 hasAuthorship W2226089114A5066217077 @default.
• W2226089114 hasAuthorship W2226089114A5074903646 @default.
• W2226089114 hasBestOaLocation W22260891142 @default.
• W2226089114 hasConcept C119062480 @default.
• W2226089114 hasConcept C153911025 @default.
• W2226089114 hasConcept C158617107 @default.
• W2226089114 hasConcept C181199279 @default.
• W2226089114 hasConcept C185592680 @default.
• W2226089114 hasConcept C2778163477 @default.
• W2226089114 hasConcept C2910033705 @default.
• W2226089114 hasConcept C41625074 @default.
• W2226089114 hasConcept C55493867 @default.
• W2226089114 hasConcept C62746215 @default.
• W2226089114 hasConcept C6492254 @default.
• W2226089114 hasConcept C79879829 @default.
• W2226089114 hasConcept C86803240 @default.
• W2226089114 hasConcept C87644729 @default.
• W2226089114 hasConcept C98539663 @default.
• W2226089114 hasConceptScore W2226089114C119062480 @default.
• W2226089114 hasConceptScore W2226089114C153911025 @default.
• W2226089114 hasConceptScore W2226089114C158617107 @default.
• W2226089114 hasConceptScore W2226089114C181199279 @default.
• W2226089114 hasConceptScore W2226089114C185592680 @default.
• W2226089114 hasConceptScore W2226089114C2778163477 @default.
• W2226089114 hasConceptScore W2226089114C2910033705 @default.
• W2226089114 hasConceptScore W2226089114C41625074 @default.
• W2226089114 hasConceptScore W2226089114C55493867 @default.
• W2226089114 hasConceptScore W2226089114C62746215 @default.
• W2226089114 hasConceptScore W2226089114C6492254 @default.
• W2226089114 hasConceptScore W2226089114C79879829 @default.
• W2226089114 hasConceptScore W2226089114C86803240 @default.
• W2226089114 hasConceptScore W2226089114C87644729 @default.
• W2226089114 hasConceptScore W2226089114C98539663 @default.
• W2226089114 hasIssue "2" @default.
• W2226089114 hasLocation W22260891141 @default.
• W2226089114 hasLocation W22260891142 @default.
• W2226089114 hasLocation W22260891143 @default.
• W2226089114 hasLocation W22260891144 @default.
• W2226089114 hasOpenAccess W2226089114 @default.
• W2226089114 hasPrimaryLocation W22260891141 @default.
• W2226089114 hasRelatedWork W1940347886 @default.
• W2226089114 hasRelatedWork W1989704540 @default.
• W2226089114 hasRelatedWork W1994803296 @default.

• next