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- W2226809396 abstract "An isolate of Fusarium sp. was obtained from seed coat of watermelon (cvs. Jingxin No.1 and Xinjingxin No.1) using seed washing test and PDA plate methods. The isolate was identified through morphological observation and molecular detection. Effects on the seed germination were also studied. After observation of morphology, color and growth rate of the colonies, and morphology of macroconidia, microconidia, chlamydospores and conidiophores, it was preliminarily identified as Fusarium oxysporum. DNA of the fungus was amplified by universal primers of fungal rDNA ITS, specific primers of genus Fusarium rDNA ITS and specific primers of F. oxysporum rDNA ITS, respectively. After PCR products were ligated, transformed and confirmed, inserted sequences of the recombinant plasmids were analyzed. The sequencing results were logged in GenBank to BLAST. The results of molecular detection and morphologic observation all showed that the Fusarium isolate should be F. oxysporum. It is the first report of seedborne F. oxysporum isolated from seed coat of watermelon in China. Moreover, the germination energy, percentage germination and germination index of watermelon seeds all decreased significantly after treated with the spore suspension of seedborne F. oxysporum." @default.
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- W2226809396 date "2009-01-01" @default.
- W2226809396 modified "2023-09-24" @default.
- W2226809396 title "Morphological and molecular identification of seedborne Fusarium of watermelon and its effect on seed germination." @default.
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