Matches in SemOpenAlex for { <https://semopenalex.org/work/W2227241519> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2227241519 endingPage "16" @default.
- W2227241519 startingPage "9" @default.
- W2227241519 abstract "The present paper reports the production, purification and biochemical characterization of an extracellular alkaline protease from Bacillus sp. SB12. The enzyme has been used as an alternative to conventional chemicals treatment for dehairing of goat skins. The protease was optimally active at 37 °C and pH 9. Starch at 2% (w/v) was used as the best carbon source and the addition of yeast extract and peptone at 1% each supported the maximum level of protease production in the presence of 5 mM Ca2+. Protease purification was performed with ammonium sulphate precipitation at 70% saturated fraction followed by dialysis and gel filtration chromatography using Sephadex G-100. The purified enzyme was homogeneous on non-denaturing PAGE and appeared as a single band with an apparent molecular weight of 41 kDa. This enzyme was moderately thermostable and has a wide pH stability range extending from pH 7 to 11. It showed high tolerance toward surfactants agents and organic solvents while it was completely inhibited by PMSF indicating the serine protease type. Purified protease was used to remove hair from goat skin proving its potential application in leather processing industry. The results revealed that the protease has enhanced the quality and physico-chemical properties of the skins while reducing the pollution." @default.
- W2227241519 created "2016-06-24" @default.
- W2227241519 creator A5034140841 @default.
- W2227241519 creator A5040613025 @default.
- W2227241519 creator A5056347256 @default.
- W2227241519 date "2016-05-01" @default.
- W2227241519 modified "2023-10-11" @default.
- W2227241519 title "Enzymatic dehairing of goat skins using alkaline protease from Bacillus sp. SB12" @default.
- W2227241519 cites W1967724527 @default.
- W2227241519 cites W1973029229 @default.
- W2227241519 cites W1975273371 @default.
- W2227241519 cites W1978432878 @default.
- W2227241519 cites W1987206719 @default.
- W2227241519 cites W1995811595 @default.
- W2227241519 cites W1996493865 @default.
- W2227241519 cites W2013648274 @default.
- W2227241519 cites W2058584290 @default.
- W2227241519 cites W2058821233 @default.
- W2227241519 cites W2061583103 @default.
- W2227241519 cites W2072549059 @default.
- W2227241519 cites W2085798465 @default.
- W2227241519 cites W2090407461 @default.
- W2227241519 cites W2093586540 @default.
- W2227241519 cites W2095402042 @default.
- W2227241519 cites W2111301002 @default.
- W2227241519 cites W2113666467 @default.
- W2227241519 cites W2115949381 @default.
- W2227241519 cites W2122932186 @default.
- W2227241519 cites W2130477345 @default.
- W2227241519 cites W2132068224 @default.
- W2227241519 cites W2166451160 @default.
- W2227241519 doi "https://doi.org/10.1016/j.pep.2015.12.021" @default.
- W2227241519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26763763" @default.
- W2227241519 hasPublicationYear "2016" @default.
- W2227241519 type Work @default.
- W2227241519 sameAs 2227241519 @default.
- W2227241519 citedByCount "27" @default.
- W2227241519 countsByYear W22272415192016 @default.
- W2227241519 countsByYear W22272415192017 @default.
- W2227241519 countsByYear W22272415192018 @default.
- W2227241519 countsByYear W22272415192019 @default.
- W2227241519 countsByYear W22272415192020 @default.
- W2227241519 countsByYear W22272415192021 @default.
- W2227241519 countsByYear W22272415192022 @default.
- W2227241519 crossrefType "journal-article" @default.
- W2227241519 hasAuthorship W2227241519A5034140841 @default.
- W2227241519 hasAuthorship W2227241519A5040613025 @default.
- W2227241519 hasAuthorship W2227241519A5056347256 @default.
- W2227241519 hasConcept C100544194 @default.
- W2227241519 hasConcept C145890019 @default.
- W2227241519 hasConcept C163994747 @default.
- W2227241519 hasConcept C181199279 @default.
- W2227241519 hasConcept C185592680 @default.
- W2227241519 hasConcept C193363816 @default.
- W2227241519 hasConcept C2776714187 @default.
- W2227241519 hasConcept C2777807008 @default.
- W2227241519 hasConcept C2780865716 @default.
- W2227241519 hasConcept C43617362 @default.
- W2227241519 hasConcept C55493867 @default.
- W2227241519 hasConceptScore W2227241519C100544194 @default.
- W2227241519 hasConceptScore W2227241519C145890019 @default.
- W2227241519 hasConceptScore W2227241519C163994747 @default.
- W2227241519 hasConceptScore W2227241519C181199279 @default.
- W2227241519 hasConceptScore W2227241519C185592680 @default.
- W2227241519 hasConceptScore W2227241519C193363816 @default.
- W2227241519 hasConceptScore W2227241519C2776714187 @default.
- W2227241519 hasConceptScore W2227241519C2777807008 @default.
- W2227241519 hasConceptScore W2227241519C2780865716 @default.
- W2227241519 hasConceptScore W2227241519C43617362 @default.
- W2227241519 hasConceptScore W2227241519C55493867 @default.
- W2227241519 hasLocation W22272415191 @default.
- W2227241519 hasLocation W22272415192 @default.
- W2227241519 hasOpenAccess W2227241519 @default.
- W2227241519 hasPrimaryLocation W22272415191 @default.
- W2227241519 hasRelatedWork W1555086762 @default.
- W2227241519 hasRelatedWork W1976608269 @default.
- W2227241519 hasRelatedWork W2096725620 @default.
- W2227241519 hasRelatedWork W2146193274 @default.
- W2227241519 hasRelatedWork W2227241519 @default.
- W2227241519 hasRelatedWork W2365177289 @default.
- W2227241519 hasRelatedWork W2404832601 @default.
- W2227241519 hasRelatedWork W2559526692 @default.
- W2227241519 hasRelatedWork W813546153 @default.
- W2227241519 hasRelatedWork W2185504084 @default.
- W2227241519 hasVolume "121" @default.
- W2227241519 isParatext "false" @default.
- W2227241519 isRetracted "false" @default.
- W2227241519 magId "2227241519" @default.
- W2227241519 workType "article" @default.