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- W2227256105 abstract "Plant tissues contain a wide range of proteins, which vary greatly in their properties, and require specific conditions for their extraction and purification. It is therefore not possible to recommend a single protocol for extraction of all plant proteins. Plant tissues do pose specific problems that must be taken into account when developing protocols for extraction. The first is the presence of a rigid cellulosic cell wall, which must be sheared to release the cell contents. The second is the presence of specific contaminating compounds that may result in protein degradation or modification and, where the protein of interest is an enzyme, subsequent loss of catalytic activity. Such compounds include phenolics and a range of proteases. Although it is sometimes possible to avoid these problems or partially control them by using a specific tissue or plant species, in other cases (for example, enzymes involved in secondary product synthesis), this is not possible and the biochemist must find ways to remove or inactivate active contaminants. The removal of phenolics is dealt with elsewhere in this volume ( Chapter 8 ). Because many plant proteases are of the serine type, it is often convenient to include the serine protease inhibitor phenylmethylsulfonylfluoride (PMSF) in extraction buffers on a routine basis (see Chapter 9 for a general discussion of protease inhibition)." @default.
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- W2227256105 date "2003-11-14" @default.
- W2227256105 modified "2023-09-25" @default.
- W2227256105 title "Protein Extraction from Plant Tissues" @default.
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- W2227256105 doi "https://doi.org/10.1385/0-89603-336-8:23" @default.
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