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- W2227261609 endingPage "6218" @default.
- W2227261609 startingPage "6205" @default.
- W2227261609 abstract "The glucosinolate biosynthetic gene AOP2 encodes an enzyme that plays a crucial role in catalysing the conversion of beneficial glucosinolates into anti-nutritional ones. In Brassica rapa, three copies of BrAOP2 have been identified, but their function in establishing the glucosinolate content of B. rapa is poorly understood. Here, we used phylogenetic and gene structure analyses to show that BrAOP2 proteins have evolved via a duplication process retaining two highly conserved domains at the N-terminal and C-terminal regions, while the middle part has experienced structural divergence. Heterologous expression and in vitro enzyme assays and Arabidopsis mutant complementation studies showed that all three BrAOP2 genes encode functional BrAOP2 proteins that convert the precursor methylsulfinyl alkyl glucosinolate to the alkenyl form. Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1). Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes. Quantitative real-time reverse transcription-PCR assays demonstrated that BrAOP2.1 showed a slightly different pattern of expression in below-ground tissue at the seedling stage and in the silique at the reproductive stage compared with BrAOP2.2 and BrAOP2.3 genes in B. rapa. Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged." @default.
- W2227261609 created "2016-06-24" @default.
- W2227261609 creator A5002250184 @default.
- W2227261609 creator A5010541585 @default.
- W2227261609 creator A5042251752 @default.
- W2227261609 creator A5051081214 @default.
- W2227261609 creator A5068670497 @default.
- W2227261609 creator A5072552402 @default.
- W2227261609 date "2015-07-17" @default.
- W2227261609 modified "2023-10-16" @default.
- W2227261609 title "Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in<i>Brassica rapa</i>" @default.
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- W2227261609 doi "https://doi.org/10.1093/jxb/erv331" @default.
- W2227261609 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4588880" @default.
- W2227261609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26188204" @default.
- W2227261609 hasPublicationYear "2015" @default.