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- W2227287787 abstract "Background: It has become increasingly clear that γδ T cells are important components in both innate and adaptive immune systems, yet the cellular requirement for the activation of γδ T cells is still poorly defined. Like αβ T cells, human Vγ9Vδ2 T cells may be divided into four subsets: naive, central memory, effector memory and terminal differentiated cells, according to their surface expression of CD45RA and CD27. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. Methods: Here we applied fluA virus-infected primary human monocyte-derived macrophages (MDMs) for examining the antiviral activity of phosphoantigen isopentenyl pyrophosphate (IPP)-expanded human Vγ9Vδ2 cells against influenza viruses. Different γδ T subsets were sorted with FACS Aria-II dependent on the surface expression levels of CD27 or CD56. Flow cytometry was used for phenotyping, cytotoxic assay and cytokine quantification. Specific blocking antibodies were utilized to uncover the mechanisms for γδ-T-mediated cytolytic antiviral effects. Results: In this study, we demonstrated that both central (CD45RA-CD27+) and effector (CD45RA-CD27-) memory Vγ9Vδ2 T had similar levels of immediate IFN-γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56+ Vγ9Vδ2 T cells had significantly higher cytotoxicity against fluA virus-infected cells, compared with CD56- counterparts, while both subsets had similar IFN-γ responses. We further demonstrated that the CD16-dependant degranulation pathway, but not antibodydependent cell-mediated cytotoxicity (ADCC), contributed to the superior cytotoxicity of CD56+ Vγ9Vδ2 T cells. Conclusion: Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T subsets during fluA virus infection, and may help improve the γδ T cell-based immunotherapy for viral infection. As γδ T cell-based immunotherapy has been showed a great potential for treating fluA infection, our findings may help improve its efficacy for the control of viral infection." @default.
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- W2227287787 date "2012-06-01" @default.
- W2227287787 modified "2023-10-01" @default.
- W2227287787 title "Phenotypic and functional characterization of human γδ T cell subsets in response to influenza A viruses" @default.
- W2227287787 doi "https://doi.org/10.1016/j.ijid.2012.05.238" @default.
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