FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2227442317> ?p ?o ?g. }

• W2227442317 endingPage "1713" @default.
• W2227442317 startingPage "1706" @default.
• W2227442317 abstract "Objective Netrin-1 is a chemorepulsant and matrix protein expressed during and required for osteoclast differentiation, which also plays a role in inflammation by preventing macrophage egress. Because wear particle-induced osteolysis requires osteoclast-mediated destruction of bone, we hypothesised that blockade of Netrin-1 or Unc5b, a receptor for Netrin-1, may diminish this pathological condition. Methods C57BL/6 mice, 6-8 weeks old, had 3 mg of ultrahigh-molecular-weight polyethylene particles implanted over the calvaria and then received 10 µg of monoclonal antibodies for Netrin-1 or its receptors, Unc5b and deleted in colon cancer (DCC), injected intraperitoneally on a weekly basis. After 2 weeks, micro-computed tomography and histology analysis were performed. Netrin-1 expression was analysed in human tissue obtained following primary prosthesis implantation or after prosthesis revision for peri-implant osteolysis and aseptic implant loosening. Results Weekly injection of anti-Netrin-1 or anti-Unc5b-antibodies significantly reduced particle-induced bone pitting in calvaria exposed to wear particles (46±4% and 49±3% of control bone pitting, respectively, p<0.001) but anti-DCC antibody did not affect inflammatory osteolysis (80±7% of control bone pitting, p=ns). Anti-Netrin-1 or anti-Unc5b, but not anti-DCC, antibody treatment markedly reduced the inflammatory infiltrate and the number of tartrate resistance acid phosphatase (TRAP)-positive osteoclasts (7±1, 4±1 and 14±1 cells/high power field (hpf), respectively, vs 12±1 cells/hpf for control, p<0.001), with no significant changes in alkaline phosphatase-positive osteoblasts on bone-forming surfaces in any antibody-treated group. Netrin-1 immunostaining colocalised with CD68 staining for macrophages. The peri-implant tissues of patients undergoing prosthesis revision surgery showed an increase in Netrin-1 expression, whereas there was little Netrin-1 expression in soft tissues removed at the time of primary joint replacement. Conclusions These results demonstrate a unique role for Netrin-1 in osteoclast biology and inflammation and may be a novel target for prevention/treatment of inflammatory osteolysis." @default.
• W2227442317 created "2016-06-24" @default.
• W2227442317 creator A5008601698 @default.
• W2227442317 creator A5018947731 @default.
• W2227442317 creator A5035258831 @default.
• W2227442317 creator A5043455810 @default.
• W2227442317 creator A5059516590 @default.
• W2227442317 creator A5076897207 @default.
• W2227442317 creator A5080984126 @default.
• W2227442317 creator A5081758402 @default.
• W2227442317 creator A5084262507 @default.
• W2227442317 date "2015-10-09" @default.
• W2227442317 modified "2023-10-14" @default.
• W2227442317 title "Netrin-1 is highly expressed and required in inflammatory infiltrates in wear particle-induced osteolysis" @default.
• W2227442317 cites W1573984805 @default.
• W2227442317 cites W1831604303 @default.
• W2227442317 cites W1846925080 @default.
• W2227442317 cites W1885826309 @default.
• W2227442317 cites W1903215685 @default.
• W2227442317 cites W1940147302 @default.
• W2227442317 cites W1973828032 @default.
• W2227442317 cites W1983009944 @default.
• W2227442317 cites W1990028015 @default.
• W2227442317 cites W1991164404 @default.
• W2227442317 cites W1997073415 @default.
• W2227442317 cites W2008299531 @default.
• W2227442317 cites W2015111630 @default.
• W2227442317 cites W2021493915 @default.
• W2227442317 cites W2029614004 @default.
• W2227442317 cites W2029920356 @default.
• W2227442317 cites W2044161694 @default.
• W2227442317 cites W2051239218 @default.
• W2227442317 cites W2056729842 @default.
• W2227442317 cites W2064342455 @default.
• W2227442317 cites W2090344258 @default.
• W2227442317 cites W2094062221 @default.
• W2227442317 cites W2105558032 @default.
• W2227442317 cites W2114119367 @default.
• W2227442317 cites W2117258058 @default.
• W2227442317 cites W2131109936 @default.
• W2227442317 cites W2131851678 @default.
• W2227442317 cites W2132070490 @default.
• W2227442317 cites W2139090780 @default.
• W2227442317 cites W2145858156 @default.
• W2227442317 cites W2151699693 @default.
• W2227442317 cites W2155407923 @default.
• W2227442317 cites W2156516646 @default.
• W2227442317 cites W2158193380 @default.
• W2227442317 cites W2162294258 @default.
• W2227442317 cites W2415048285 @default.
• W2227442317 cites W4249814361 @default.
• W2227442317 doi "https://doi.org/10.1136/annrheumdis-2015-207593" @default.
• W2227442317 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5349296" @default.
• W2227442317 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26452536" @default.
• W2227442317 hasPublicationYear "2015" @default.
• W2227442317 type Work @default.
• W2227442317 sameAs 2227442317 @default.
• W2227442317 citedByCount "24" @default.
• W2227442317 countsByYear W22274423172016 @default.
• W2227442317 countsByYear W22274423172017 @default.
• W2227442317 countsByYear W22274423172018 @default.
• W2227442317 countsByYear W22274423172019 @default.
• W2227442317 countsByYear W22274423172020 @default.
• W2227442317 countsByYear W22274423172021 @default.
• W2227442317 countsByYear W22274423172022 @default.
• W2227442317 crossrefType "journal-article" @default.
• W2227442317 hasAuthorship W2227442317A5008601698 @default.
• W2227442317 hasAuthorship W2227442317A5018947731 @default.
• W2227442317 hasAuthorship W2227442317A5035258831 @default.
• W2227442317 hasAuthorship W2227442317A5043455810 @default.
• W2227442317 hasAuthorship W2227442317A5059516590 @default.
• W2227442317 hasAuthorship W2227442317A5076897207 @default.
• W2227442317 hasAuthorship W2227442317A5080984126 @default.
• W2227442317 hasAuthorship W2227442317A5081758402 @default.
• W2227442317 hasAuthorship W2227442317A5084262507 @default.
• W2227442317 hasBestOaLocation W22274423172 @default.
• W2227442317 hasConcept C126322002 @default.
• W2227442317 hasConcept C134018914 @default.
• W2227442317 hasConcept C141071460 @default.
• W2227442317 hasConcept C142724271 @default.
• W2227442317 hasConcept C170493617 @default.
• W2227442317 hasConcept C180675623 @default.
• W2227442317 hasConcept C181199279 @default.
• W2227442317 hasConcept C185592680 @default.
• W2227442317 hasConcept C202751555 @default.
• W2227442317 hasConcept C21547065 @default.
• W2227442317 hasConcept C2776033226 @default.
• W2227442317 hasConcept C2776914184 @default.
• W2227442317 hasConcept C2779918246 @default.
• W2227442317 hasConcept C2781282405 @default.
• W2227442317 hasConcept C2781411149 @default.
• W2227442317 hasConcept C55493867 @default.
• W2227442317 hasConcept C673006 @default.
• W2227442317 hasConcept C71924100 @default.
• W2227442317 hasConceptScore W2227442317C126322002 @default.
• W2227442317 hasConceptScore W2227442317C134018914 @default.
• W2227442317 hasConceptScore W2227442317C141071460 @default.
• W2227442317 hasConceptScore W2227442317C142724271 @default.

• next