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- W2227607047 abstract "To clarify some of the controversial aspects of immune responses ascribed to isocyanates both in exposed workers and in laboratory animals, this investigation sought to establish an analogous homocytotropic antibody model in mice immunized with well-characterized isocyanate-protein conjugates. Toluene diisocyanate was conjugated to serum albumins of human, bovine, and ovine species under controlled conditions, and the amount of ligand bound to various proteins was determined spectrophotometrically. Strain B6D2F1 mice were immunized and boosted 28 days later with 100 μg of toluene diisocyanate-conjugated proteins or unconjugated proteins plus 1 mg of alum. Sera were assessed for 48-h passive cutaneous anaphylaxis in hairless mice. Sera from mice immunized with toluene diisocyanate-conjugated human, bovine, or ovine serum albumin demonstrated passive cutaneous anaphylaxis titers of 1:57 or higher when challenged with homologous conjugates and somewhat lower titers (range, <1:5 to 1:20) after reactions with ..." @default.
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- W2227607047 date "2015-05-14" @default.
- W2227607047 modified "2023-09-24" @default.
- W2227607047 title "Induction of Murine Reaginic Antibodies by Toluene Diisocyanate" @default.
- W2227607047 hasPublicationYear "2015" @default.
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