Matches in SemOpenAlex for { <https://semopenalex.org/work/W2227696295> ?p ?o ?g. }
- W2227696295 endingPage "101" @default.
- W2227696295 startingPage "87" @default.
- W2227696295 abstract "Magic angle spinning (MAS) solid-state nuclear magnetic resonance (NMR) is a powerful method for structure determination of insoluble biomolecules. However, structure determination by MAS solid-state NMR remains challenging because it is difficult to obtain a sufficient amount of distance restraints owing to spectral complexity. Collection of distance restraints from paramagnetic relaxation enhancement (PRE) is a promising approach to alleviate this barrier. However, the precision of distance restraints provided by PRE is limited in solid-state NMR because of incomplete averaged interactions and intermolecular PREs. In this report, the backbone structure of the B1 domain of streptococcal protein G (GB1) has been successfully determined by combining the CS-Rosetta protocol and qualitative PRE restraints. The derived structure has a Cα RMSD of 1.49 Å relative to the X-ray structure. It is noteworthy that our protocol can determine the correct structure from only three cysteine-EDTA-Mn(2+) mutants because this number of PRE sites is insufficient when using a conventional structure calculation method based on restrained molecular dynamics and simulated annealing. This study shows that qualitative PRE restraints can be employed effectively for protein structure determination from a limited conformational sampling space using a protein fragment library." @default.
- W2227696295 created "2016-06-24" @default.
- W2227696295 creator A5010059929 @default.
- W2227696295 creator A5021912526 @default.
- W2227696295 creator A5044279513 @default.
- W2227696295 creator A5057541087 @default.
- W2227696295 creator A5067444942 @default.
- W2227696295 creator A5084464179 @default.
- W2227696295 creator A5084765225 @default.
- W2227696295 creator A5088528790 @default.
- W2227696295 creator A5090671504 @default.
- W2227696295 date "2016-01-01" @default.
- W2227696295 modified "2023-09-26" @default.
- W2227696295 title "Structure determination of uniformly 13C, 15N labeled protein using qualitative distance restraints from MAS solid-state 13C-NMR observed paramagnetic relaxation enhancement" @default.
- W2227696295 cites W1548178570 @default.
- W2227696295 cites W1963525648 @default.
- W2227696295 cites W1966420704 @default.
- W2227696295 cites W1966910457 @default.
- W2227696295 cites W1980661677 @default.
- W2227696295 cites W1981331687 @default.
- W2227696295 cites W1992638791 @default.
- W2227696295 cites W1993850177 @default.
- W2227696295 cites W1994277766 @default.
- W2227696295 cites W2000948557 @default.
- W2227696295 cites W2002172527 @default.
- W2227696295 cites W2002869713 @default.
- W2227696295 cites W2003041358 @default.
- W2227696295 cites W2003560901 @default.
- W2227696295 cites W2004461664 @default.
- W2227696295 cites W2004802356 @default.
- W2227696295 cites W2005801417 @default.
- W2227696295 cites W2006422372 @default.
- W2227696295 cites W2006449277 @default.
- W2227696295 cites W2007423436 @default.
- W2227696295 cites W2011148270 @default.
- W2227696295 cites W2012269679 @default.
- W2227696295 cites W2023386860 @default.
- W2227696295 cites W2036154453 @default.
- W2227696295 cites W2038090296 @default.
- W2227696295 cites W2038853976 @default.
- W2227696295 cites W2040221508 @default.
- W2227696295 cites W2041275398 @default.
- W2227696295 cites W2047865819 @default.
- W2227696295 cites W2048266405 @default.
- W2227696295 cites W2049457832 @default.
- W2227696295 cites W2051107932 @default.
- W2227696295 cites W2077819363 @default.
- W2227696295 cites W2086565576 @default.
- W2227696295 cites W2087542083 @default.
- W2227696295 cites W2089227855 @default.
- W2227696295 cites W2091670229 @default.
- W2227696295 cites W2091704437 @default.
- W2227696295 cites W2092024874 @default.
- W2227696295 cites W2094150460 @default.
- W2227696295 cites W2094386152 @default.
- W2227696295 cites W2097897519 @default.
- W2227696295 cites W2110346643 @default.
- W2227696295 cites W2110461335 @default.
- W2227696295 cites W2112418305 @default.
- W2227696295 cites W2127131680 @default.
- W2227696295 cites W2143434019 @default.
- W2227696295 cites W2164113581 @default.
- W2227696295 cites W2164664568 @default.
- W2227696295 cites W2169821755 @default.
- W2227696295 cites W2170671445 @default.
- W2227696295 cites W2201713963 @default.
- W2227696295 cites W2325864946 @default.
- W2227696295 cites W4242009576 @default.
- W2227696295 cites W886277281 @default.
- W2227696295 doi "https://doi.org/10.1007/s10858-015-0010-0" @default.
- W2227696295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26728076" @default.
- W2227696295 hasPublicationYear "2016" @default.
- W2227696295 type Work @default.
- W2227696295 sameAs 2227696295 @default.
- W2227696295 citedByCount "23" @default.
- W2227696295 countsByYear W22276962952017 @default.
- W2227696295 countsByYear W22276962952018 @default.
- W2227696295 countsByYear W22276962952019 @default.
- W2227696295 countsByYear W22276962952020 @default.
- W2227696295 countsByYear W22276962952021 @default.
- W2227696295 countsByYear W22276962952022 @default.
- W2227696295 crossrefType "journal-article" @default.
- W2227696295 hasAuthorship W2227696295A5010059929 @default.
- W2227696295 hasAuthorship W2227696295A5021912526 @default.
- W2227696295 hasAuthorship W2227696295A5044279513 @default.
- W2227696295 hasAuthorship W2227696295A5057541087 @default.
- W2227696295 hasAuthorship W2227696295A5067444942 @default.
- W2227696295 hasAuthorship W2227696295A5084464179 @default.
- W2227696295 hasAuthorship W2227696295A5084765225 @default.
- W2227696295 hasAuthorship W2227696295A5088528790 @default.
- W2227696295 hasAuthorship W2227696295A5090671504 @default.
- W2227696295 hasBestOaLocation W22276962952 @default.
- W2227696295 hasConcept C121332964 @default.
- W2227696295 hasConcept C124712363 @default.
- W2227696295 hasConcept C153202636 @default.
- W2227696295 hasConcept C15744967 @default.
- W2227696295 hasConcept C166950319 @default.
- W2227696295 hasConcept C178790620 @default.