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- W2227758546 abstract "[Growth factors are regulatory peptides involved in many processes of cell physiology and pathology, including tissue repair and tumor growth. Part of this work concerned the development of a genetically engineered biological bandage (GEBB) designed to improve wound healing. Human epidermal keratinocytes (SCC-13) were genetically engineered to produce bovine growth hormone (bGH), giving raise to higher producer bGH cell lines (1 ug/ 1 {dollar}{bsol}times{dollar} 10{dollar}{bsol}sp6{dollar} cells/24hs). Selection of biocompatible polymers for bandage composition and appropriate conditions for cell maintenance was also established. The GEBB consists of a hydrophylic and gas permeable top and bottom membranes (Z-bind) united by a gasket. The bottom membrane allows cell attachment and growth, and diffusion of macromolecules. This system was functional under ambient conditions; when placed onto a full-thickness, surgically generated wound on rats, the cells within the bandage released bGH for three days. The GEBB represents a versatile system for delivering a variety of biological agents to wounds. In the second part of these studies, the role of retinoic acid (RA) and epidermal growth factor (EGF) in the regulation of insulin-like growth factor binding protein-3 (IGFBP-3) was addressed. IGFBP-3 regulates insulin-like gr owth factors (IGFs) which in turn are important modulators of epithelium proliferation. Treatment of the ectocervical epithelial cell line ECE16-1 with EGF caused a marked reduction in IGFBP-3 levels. In contrast, RA increased mRNA and protein levels in the presence or absence of EGF. This response was concentration-dependent with a half-maximal increase observed at 1 nM RA. RA was able to reverse the EGF supression in IGFBP-3 when added simultaneously or three days after EGF treatment. Conversely, when ECE16-1 were treated with RA, IGFBP-3 levels increased within 24 h and subsequent addition of EGF was without effect. Thus, the RA-dependent increase in IGFBP-3 is dominant over the EGF supression. Increased IGFBP-3 levels correlated with supressed growth. RA also increased IGFBP-3 mRNA in other ectocervical cell lines. These results suggest that RA may act to inhibit ectocervical proliferation by increasing IGFBP-3 levels and/or reducing the extracellular concentration of free IGFs]" @default.
- W2227758546 created "2016-06-24" @default.
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- W2227758546 date "1994-01-01" @default.
- W2227758546 modified "2023-09-23" @default.
- W2227758546 title "Experimental approaches for enhancing wound healing and inhibiting tumor growth" @default.
- W2227758546 hasPublicationYear "1994" @default.
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