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- W2227930129 abstract "Publisher Summary This chapter discusses the separation of malignant cells from host cells using centrifugal elutriation. Several cell separation procedures have been developed for the purification of in situ host cells from tumor cells. The chapter reviews the application of centrifugal elutriation as a procedure for the isolation of different cell subpopulations from tumor tissue in situ. In the process of cell separation by centrifugal elutriation, cells suspended in the separation chamber of an elutriator rotor are subjected to two opposite forces: the centrifugal force generated from the rotation of the rotor in an outward direction and the fluid force through the chamber in an inward or centripetal direction. The special shape of the separation chamber makes it possible to generate a gradient of flow rate, decreasing toward the center of rotation. Various practical aspects of the separation involved in the method of centrifugal elutriation can influence the homogeneity of cell subpopulations. The chapter also discusses those factors that are directly relevant to the separation of in situ tumor cells. Some of these factors include cell volume distributions between the host and malignant cells, effects of tumor dissociation on the quality and quantity of single-cell suspensions, and the modification of collection methods to improve the homogeneity." @default.
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- W2227930129 date "1987-01-01" @default.
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- W2227930129 title "Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation" @default.
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- W2227930129 doi "https://doi.org/10.1016/b978-0-12-564505-8.50007-1" @default.
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