FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2228176311> ?p ?o ?g. }

• W2228176311 abstract "We investigated the mechanism by which the G-protein activators aluminium fluoride and vanadate stimulate arachidonic acid release in pig aortic endothelial cells. Our previous study demonstrated a novel Ca(2+)-independent pathway of phospholipase A2 (PLA2) activation stimulated by aluminium fluoride in this model. In the present study, we found that sodium metavanadate stimulated a rapid concentration-dependent release of [3H]arachidonic acid from prelabelled cells. A more than 3-fold enhancement of arachidonic acid release was achieved in cells treated with 1 mM vanadate for 20 min. Synthesis of prostaglandin products was similarly enhanced. The release of arachidonic acid was not dependent on the presence of extracellular Ca2+, but did require protein synthesis de novo. Both cycloheximide and actinomycin D completely blocked aluminium fluoride- and vanadate-stimulated arachidonic acid release. Because fluoride and vanadate are known protein tyrosine phosphatase inhibitors, it is possible that PLA2 activation occurred secondarily to changes in protein tyrosine phosphorylation. Both aluminium fluoride and vanadate stimulated the rapid phosphorylation of 58, 93 and 120 kDa tyrosine-containing protein substrates. However, in contrast with arachidonic acid release, this response was found to be sensitive to the presence of extracellular Ca2+ and insensitive to blockers of protein synthesis de novo. Furthermore H2O2 treatment resulted in rapid tyrosine phosphorylation of the same substrates without a concomitant increase in arachidonic acid release. These results suggest that the effects of aluminium fluoride and vanadate on PLA2 are not due to changes in protein tyrosine phosphorylation, but do require rapid protein synthesis de novo." @default.
• W2228176311 created "2016-06-24" @default.
• W2228176311 creator A5027071944 @default.
• W2228176311 creator A5030418423 @default.
• W2228176311 date "1994-06-01" @default.
• W2228176311 modified "2023-09-25" @default.
• W2228176311 title "Ca2+-independent arachidonic acid release by vascular endothelium requires protein synthesis de novo" @default.
• W2228176311 cites W1036181247 @default.
• W2228176311 cites W1482582564 @default.
• W2228176311 cites W1484647033 @default.
• W2228176311 cites W1517887854 @default.
• W2228176311 cites W1544929992 @default.
• W2228176311 cites W1560653093 @default.
• W2228176311 cites W1561795287 @default.
• W2228176311 cites W1569244310 @default.
• W2228176311 cites W1569457616 @default.
• W2228176311 cites W1583075434 @default.
• W2228176311 cites W1585634514 @default.
• W2228176311 cites W1587929792 @default.
• W2228176311 cites W16075687 @default.
• W2228176311 cites W1641540149 @default.
• W2228176311 cites W1659855560 @default.
• W2228176311 cites W1659985814 @default.
• W2228176311 cites W1668450698 @default.
• W2228176311 cites W1696959307 @default.
• W2228176311 cites W1776295853 @default.
• W2228176311 cites W1947815351 @default.
• W2228176311 cites W1962519158 @default.
• W2228176311 cites W1966177328 @default.
• W2228176311 cites W1979591208 @default.
• W2228176311 cites W1993378893 @default.
• W2228176311 cites W1994928222 @default.
• W2228176311 cites W2022037486 @default.
• W2228176311 cites W2022185868 @default.
• W2228176311 cites W2026036438 @default.
• W2228176311 cites W2029212579 @default.
• W2228176311 cites W2032359283 @default.
• W2228176311 cites W2044500147 @default.
• W2228176311 cites W2050519601 @default.
• W2228176311 cites W2051958752 @default.
• W2228176311 cites W2052295727 @default.
• W2228176311 cites W2058440862 @default.
• W2228176311 cites W2063900196 @default.
• W2228176311 cites W2082611407 @default.
• W2228176311 cites W2087262804 @default.
• W2228176311 cites W2095080714 @default.
• W2228176311 cites W2255027740 @default.
• W2228176311 cites W325602451 @default.
• W2228176311 doi "https://doi.org/10.1042/bj3000449" @default.
• W2228176311 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1138183" @default.
• W2228176311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8002950" @default.
• W2228176311 hasPublicationYear "1994" @default.
• W2228176311 type Work @default.
• W2228176311 sameAs 2228176311 @default.
• W2228176311 citedByCount "9" @default.
• W2228176311 crossrefType "journal-article" @default.
• W2228176311 hasAuthorship W2228176311A5027071944 @default.
• W2228176311 hasAuthorship W2228176311A5030418423 @default.
• W2228176311 hasBestOaLocation W22281763112 @default.
• W2228176311 hasConcept C11960822 @default.
• W2228176311 hasConcept C179104552 @default.
• W2228176311 hasConcept C181199279 @default.
• W2228176311 hasConcept C185592680 @default.
• W2228176311 hasConcept C2776165026 @default.
• W2228176311 hasConcept C2777553839 @default.
• W2228176311 hasConcept C2778078955 @default.
• W2228176311 hasConcept C2779234887 @default.
• W2228176311 hasConcept C2780828025 @default.
• W2228176311 hasConcept C2781116636 @default.
• W2228176311 hasConcept C55493867 @default.
• W2228176311 hasConcept C85528070 @default.
• W2228176311 hasConcept C86803240 @default.
• W2228176311 hasConcept C87325107 @default.
• W2228176311 hasConcept C97029542 @default.
• W2228176311 hasConceptScore W2228176311C11960822 @default.
• W2228176311 hasConceptScore W2228176311C179104552 @default.
• W2228176311 hasConceptScore W2228176311C181199279 @default.
• W2228176311 hasConceptScore W2228176311C185592680 @default.
• W2228176311 hasConceptScore W2228176311C2776165026 @default.
• W2228176311 hasConceptScore W2228176311C2777553839 @default.
• W2228176311 hasConceptScore W2228176311C2778078955 @default.
• W2228176311 hasConceptScore W2228176311C2779234887 @default.
• W2228176311 hasConceptScore W2228176311C2780828025 @default.
• W2228176311 hasConceptScore W2228176311C2781116636 @default.
• W2228176311 hasConceptScore W2228176311C55493867 @default.
• W2228176311 hasConceptScore W2228176311C85528070 @default.
• W2228176311 hasConceptScore W2228176311C86803240 @default.
• W2228176311 hasConceptScore W2228176311C87325107 @default.
• W2228176311 hasConceptScore W2228176311C97029542 @default.
• W2228176311 hasLocation W22281763111 @default.
• W2228176311 hasLocation W22281763112 @default.
• W2228176311 hasLocation W22281763113 @default.
• W2228176311 hasLocation W22281763114 @default.
• W2228176311 hasOpenAccess W2228176311 @default.
• W2228176311 hasPrimaryLocation W22281763111 @default.
• W2228176311 hasRelatedWork W1546164822 @default.
• W2228176311 hasRelatedWork W1562710579 @default.
• W2228176311 hasRelatedWork W1659855560 @default.
• W2228176311 hasRelatedWork W1880220804 @default.
• W2228176311 hasRelatedWork W1983058690 @default.

• next