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- W2228558275 abstract "Objective:To investigate extraction and ultraviolet spectrophotometric determination of polysaccharides in sporophore of Cordyceps militaris(L.) link.Methods: The sample was processed by extraction using water,precipitation using ethanol and purification using basic cupric sulfate.Polysaccharides,under strong acidic conditions,were hydrolyzed into monosaccharide molecules.Dehydration in a boiling water bath produced hydroxymethyl furfural,which was subjected to condensation with phenol to produce a compound with light orange color.The color intensity was in a sensitive and stable positive relationship with the concentration of monosaccharides in the solution and exhibited the maximum absorption at 485 nm in colorimetric determination.Results: Good linearity was presented between the absorbance value and the concentration of the reference substance(calculated with respect to polyglucosan) within the range of 0.0 mg/ml~0.1 mg/ml.The regression equation was y=9.7717x-0.0006(r=0.9996).The mean recovery was 97.37% and the RSD was 2.73%.Conclusion: Ultraviolet spectrophotometry is sensitive,rapid,stable and cost-effective and thus applicable to determination of polysaccharides in sporophore of Cordyceps militaris(L.) link.Different extraction methods may lead to different results." @default.
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- W2228558275 date "2011-01-01" @default.
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- W2228558275 title "A study on determination of polysaccharides in sporophore of cordyceps militaris" @default.
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