FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2228729418> ?p ?o ?g. }

• W2228729418 abstract "Using a strategy of large-scale whole mount in situ hybridization, three genes were identified from a cDNA library constructed from endoderm-like tissue induced from activin treated animal caps. One gene, XODC2, encodes a paralogue to ubiquitous ODC (Genbank accession number: AF217544); another, XCL-2, encodes a tissue-specific m-type Calpain (Genbank accession number: AF212199); while the third one, XETOR, encodes a novel member of the ETO/MTG8 oncoprotein family (Genbank accession number: AF212198). Spatial and temporal expressions of these genes were examined by ways of whole mount in situ hybridization and RT-PCR. Functional analyses were focused on XCL-2 and XETOR. Overexpression of wild-type XCL-2 suggests that this gene is involved in gastrulation movement and convergent extension during gastrulation and neurulation. Overexpression of a dominant-negative mutant caused a phenotypemorphologically similar to, but histologically different from, that caused by overexpression of wild-type XCL-2. The mutant phenotype can be rescued by injection of wild-type XCL-2. These data suggest that XCL-2 plays an important role in convergent extension movements during embryogenesis in Xenopus laevis. XETOR is expressed during neurula stage in three bilaterally symmetrical stripes at each side of dorsal midline, a pattern similar to that of the genes involved in primary neurogenesis. Indeed, overexpression of XETOR or a series of truncated mutants led to the inhibition of primary neuron formation without disruption of neural plates. Such an inhibitory effect is not mediated by lateral inhibition, but an independent action. Moreover, it was shown that XETOR and lateral inhibition antagonizes each other. Further evidence showed that expression of XETOR is activated or promoted by overexpressed proneural genes such as Xngnr-1, Xash-3, Xath3, and XNeuroD, and conversely, overexpressed XETOR inhibits the expression and function of Xath3 and XNeuroD. The neuron inducing activity but not expression of Xash-3 is inhibited in response to XETOR overexpression, while neither the expression nor the function of Xngnr-1 is inhibited. Thus a negative feedback loop is established between XETOR and proneural genes. Blocking of XETOR function in vivo resulted in a neurogenic phenotype of an enlarged neurogenic domain without alteration in neuron density. Nevertheless, double depletion of XETOR and lateral inhibition led to primary neuron formation with an increased density in enlarged domains. Based on these data, it is concluded that during primaryneurogenesis, lateral inhibition and XETOR comprise a dual inhibitory mechanism to refine the number and localization of primary neurons via repression of the expression and function of proneural genes." @default.
• W2228729418 created "2016-06-24" @default.
• W2228729418 creator A5045899901 @default.
• W2228729418 date "2002-07-02" @default.
• W2228729418 modified "2023-09-24" @default.
• W2228729418 title "Screening and characterization of novel genes involved in the embryogenesis of Xenoplus laevis" @default.
• W2228729418 hasPublicationYear "2002" @default.
• W2228729418 type Work @default.
• W2228729418 sameAs 2228729418 @default.
• W2228729418 citedByCount "0" @default.
• W2228729418 crossrefType "dissertation" @default.
• W2228729418 hasAuthorship W2228729418A5045899901 @default.
• W2228729418 hasConcept C104317684 @default.
• W2228729418 hasConcept C127716648 @default.
• W2228729418 hasConcept C129536746 @default.
• W2228729418 hasConcept C143065580 @default.
• W2228729418 hasConcept C150194340 @default.
• W2228729418 hasConcept C153911025 @default.
• W2228729418 hasConcept C192586883 @default.
• W2228729418 hasConcept C2779535977 @default.
• W2228729418 hasConcept C41623484 @default.
• W2228729418 hasConcept C54355233 @default.
• W2228729418 hasConcept C81439078 @default.
• W2228729418 hasConcept C86803240 @default.
• W2228729418 hasConcept C87073359 @default.
• W2228729418 hasConcept C95444343 @default.
• W2228729418 hasConceptScore W2228729418C104317684 @default.
• W2228729418 hasConceptScore W2228729418C127716648 @default.
• W2228729418 hasConceptScore W2228729418C129536746 @default.
• W2228729418 hasConceptScore W2228729418C143065580 @default.
• W2228729418 hasConceptScore W2228729418C150194340 @default.
• W2228729418 hasConceptScore W2228729418C153911025 @default.
• W2228729418 hasConceptScore W2228729418C192586883 @default.
• W2228729418 hasConceptScore W2228729418C2779535977 @default.
• W2228729418 hasConceptScore W2228729418C41623484 @default.
• W2228729418 hasConceptScore W2228729418C54355233 @default.
• W2228729418 hasConceptScore W2228729418C81439078 @default.
• W2228729418 hasConceptScore W2228729418C86803240 @default.
• W2228729418 hasConceptScore W2228729418C87073359 @default.
• W2228729418 hasConceptScore W2228729418C95444343 @default.
• W2228729418 hasLocation W22287294181 @default.
• W2228729418 hasOpenAccess W2228729418 @default.
• W2228729418 hasPrimaryLocation W22287294181 @default.
• W2228729418 hasRelatedWork W1709735914 @default.
• W2228729418 hasRelatedWork W1840848923 @default.
• W2228729418 hasRelatedWork W1967380741 @default.
• W2228729418 hasRelatedWork W1981788032 @default.
• W2228729418 hasRelatedWork W2005791473 @default.
• W2228729418 hasRelatedWork W2008462178 @default.
• W2228729418 hasRelatedWork W2008469161 @default.
• W2228729418 hasRelatedWork W2018507268 @default.
• W2228729418 hasRelatedWork W2031383107 @default.
• W2228729418 hasRelatedWork W2047092290 @default.
• W2228729418 hasRelatedWork W2059394768 @default.
• W2228729418 hasRelatedWork W2075559846 @default.
• W2228729418 hasRelatedWork W2086321869 @default.
• W2228729418 hasRelatedWork W2095995533 @default.
• W2228729418 hasRelatedWork W2144710208 @default.
• W2228729418 hasRelatedWork W2171623512 @default.
• W2228729418 hasRelatedWork W2771268173 @default.
• W2228729418 hasRelatedWork W2885796808 @default.
• W2228729418 hasRelatedWork W75242089 @default.
• W2228729418 hasRelatedWork W2290183477 @default.
• W2228729418 isParatext "false" @default.
• W2228729418 isRetracted "false" @default.
• W2228729418 magId "2228729418" @default.
• W2228729418 workType "dissertation" @default.