FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2228932829> ?p ?o ?g. }

• W2228932829 endingPage "1334" @default.
• W2228932829 startingPage "1327" @default.
• W2228932829 abstract "Heparanase (HPSE) and vascular endothelial growth factor C (VEGF-C) are important cytokines that promote metastasis and angiogenesis in numerous malignant neoplasms, however, their association remains unclear in pancreatic ductal cell adenocarcinoma (PDAC). The present study aimed to investigate whether HPSE has a positive correlation with VEGF‑C expression and to uncover the role it plays in the in vitro invasion of BxPC‑3 cells (a pancreatic carcinoma cell line), and to analyze the value of joint detection of HPSE and VEGF‑C for PDAC patients. A recombinant plasmid, GV230/HPSE was constructed and BxPC‑3 cells were transiently transfected with GV230/HPSE or siRNA against HPSE. The expression levels of HPSE and VEGF‑C were compared using reverse transcription quantitative PCR (RT‑qPCR) and immunoblotting. The metastatic potential of treated BxPC‑3 cells was evaluated using a Transwell® invasion assay. The relative mRNA levels of HPSE and VEGF‑C in 34 PDAC specimens were assessed by RT‑qPCR. The results of the RT‑qPCR demonstrated a 10.7‑ and 3.24‑fold elevation (P<0.01) of HPSE mRNA and VEGF‑C mRNA, respectively, in GV230/HPSE group, whereas the HPSE siRNA group were downregulated for these mRNAs (‑2.45‑fold, P<0.01; ‑1.84‑fold, P<0.01). The same pattern for protein expression was detected using immunoblot assays. In Transwell® invasion assays 138±5 cells in GV230 /HPSE group and 53±4 cells in siRNA group migrated through the Matrigel®. A negative correlation between the mRNA levels of HPSE and VEGF‑C in PDAC specimens and the prognosis factors of the postoperative patients was identified. Spearman rank correlation analysis indicated a positive correlation between HPSE and VEGF‑C in PDAC (r=0.812, P<0.01). HPSE regulates the expression of VEGF‑C and facilitates invasion of BxPC‑3 in vitro. Joint detection of HPSE and VEGF‑C may therefore be clinically useful in determining the prognosis of pancreatic cancer patients." @default.
• W2228932829 created "2016-06-24" @default.
• W2228932829 creator A5030699547 @default.
• W2228932829 creator A5031639923 @default.
• W2228932829 creator A5033393490 @default.
• W2228932829 creator A5046881277 @default.
• W2228932829 date "2016-01-08" @default.
• W2228932829 modified "2023-10-16" @default.
• W2228932829 title "Heparanase regulates in vitro VEGF-C expression and its clinical significance to pancreatic ductal cell adenocarcinoma" @default.
• W2228932829 cites W1563898076 @default.
• W2228932829 cites W1971939635 @default.
• W2228932829 cites W1973037169 @default.
• W2228932829 cites W1977455794 @default.
• W2228932829 cites W1978312159 @default.
• W2228932829 cites W1980860576 @default.
• W2228932829 cites W1985043086 @default.
• W2228932829 cites W1993147071 @default.
• W2228932829 cites W1997747049 @default.
• W2228932829 cites W2004577947 @default.
• W2228932829 cites W2018242510 @default.
• W2228932829 cites W2047876833 @default.
• W2228932829 cites W2062091919 @default.
• W2228932829 cites W2062110955 @default.
• W2228932829 cites W2070407623 @default.
• W2228932829 cites W2083200833 @default.
• W2228932829 cites W2083280176 @default.
• W2228932829 cites W2097145108 @default.
• W2228932829 cites W2101425659 @default.
• W2228932829 cites W2106545194 @default.
• W2228932829 cites W2114570899 @default.
• W2228932829 cites W2138537326 @default.
• W2228932829 cites W2139836705 @default.
• W2228932829 cites W2146148087 @default.
• W2228932829 cites W2161208672 @default.
• W2228932829 cites W2165717859 @default.
• W2228932829 cites W2166375250 @default.
• W2228932829 cites W2168420558 @default.
• W2228932829 cites W2287784331 @default.
• W2228932829 cites W2319337624 @default.
• W2228932829 cites W2399936336 @default.
• W2228932829 doi "https://doi.org/10.3892/ol.2016.4085" @default.
• W2228932829 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4734281" @default.
• W2228932829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26893739" @default.
• W2228932829 hasPublicationYear "2016" @default.
• W2228932829 type Work @default.
• W2228932829 sameAs 2228932829 @default.
• W2228932829 citedByCount "12" @default.
• W2228932829 countsByYear W22289328292016 @default.
• W2228932829 countsByYear W22289328292017 @default.
• W2228932829 countsByYear W22289328292019 @default.
• W2228932829 countsByYear W22289328292020 @default.
• W2228932829 countsByYear W22289328292021 @default.
• W2228932829 countsByYear W22289328292022 @default.
• W2228932829 countsByYear W22289328292023 @default.
• W2228932829 crossrefType "journal-article" @default.
• W2228932829 hasAuthorship W2228932829A5030699547 @default.
• W2228932829 hasAuthorship W2228932829A5031639923 @default.
• W2228932829 hasAuthorship W2228932829A5033393490 @default.
• W2228932829 hasAuthorship W2228932829A5046881277 @default.
• W2228932829 hasBestOaLocation W22289328291 @default.
• W2228932829 hasConcept C11957725 @default.
• W2228932829 hasConcept C121608353 @default.
• W2228932829 hasConcept C126322002 @default.
• W2228932829 hasConcept C1491633281 @default.
• W2228932829 hasConcept C153911025 @default.
• W2228932829 hasConcept C167734588 @default.
• W2228932829 hasConcept C2777025900 @default.
• W2228932829 hasConcept C2777478136 @default.
• W2228932829 hasConcept C2778608917 @default.
• W2228932829 hasConcept C2779013556 @default.
• W2228932829 hasConcept C2780210213 @default.
• W2228932829 hasConcept C2780394083 @default.
• W2228932829 hasConcept C2781018059 @default.
• W2228932829 hasConcept C29537977 @default.
• W2228932829 hasConcept C502942594 @default.
• W2228932829 hasConcept C54355233 @default.
• W2228932829 hasConcept C67082663 @default.
• W2228932829 hasConcept C71924100 @default.
• W2228932829 hasConcept C81885089 @default.
• W2228932829 hasConcept C86803240 @default.
• W2228932829 hasConceptScore W2228932829C11957725 @default.
• W2228932829 hasConceptScore W2228932829C121608353 @default.
• W2228932829 hasConceptScore W2228932829C126322002 @default.
• W2228932829 hasConceptScore W2228932829C1491633281 @default.
• W2228932829 hasConceptScore W2228932829C153911025 @default.
• W2228932829 hasConceptScore W2228932829C167734588 @default.
• W2228932829 hasConceptScore W2228932829C2777025900 @default.
• W2228932829 hasConceptScore W2228932829C2777478136 @default.
• W2228932829 hasConceptScore W2228932829C2778608917 @default.
• W2228932829 hasConceptScore W2228932829C2779013556 @default.
• W2228932829 hasConceptScore W2228932829C2780210213 @default.
• W2228932829 hasConceptScore W2228932829C2780394083 @default.
• W2228932829 hasConceptScore W2228932829C2781018059 @default.
• W2228932829 hasConceptScore W2228932829C29537977 @default.
• W2228932829 hasConceptScore W2228932829C502942594 @default.
• W2228932829 hasConceptScore W2228932829C54355233 @default.
• W2228932829 hasConceptScore W2228932829C67082663 @default.
• W2228932829 hasConceptScore W2228932829C71924100 @default.

• next