FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2230439594> ?p ?o ?g. }

• W2230439594 endingPage "4420" @default.
• W2230439594 startingPage "4420" @default.
• W2230439594 abstract "AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA4420 Rexinoids are important in controlling apoptosis and are known to suppress both ER-positive and ER-negative mammary tumor development with reduced toxicity compared to RAR-selective retinoids. Rexinoids are also active in animals in tamoxifen-resistant breast cancer and in ATRA-resistant breast cancer cells. Hence, rexinoids seems to be more efficient and promising chemopreventive and therapeutic drugs compare to RAR-selective ligands. Our goal of the study of the present study is to know whether LGD1069 and LG100268 (rexinoids-from Ligand Pharmaceuticals Inc.) suppress the growth of breast cancer cells, and how these drugs act to inhibit breast cancer development. For that purpose, we tested 5 different breast cell lines; 1 human mammary epithelial cell line (HMEC), 2 different ER-positive cell lines (MCF-7 and T47D) and 2 different ER-negative breast cancer cell lines (MDA-MB-231 and MDA-MB-435). By MTS assay, We found that both LGD1069 and LG100268 inhibited significantly normal HMEC cell growth at 10 uM. We also found that LGD1069 strongly suppressed the growth of T47D (ER-positive) by dose-dependent manner. LGD1069 also induced a mild inhibition of MDA-MB-231 (ER-negative) at 10 uM. MCF-7 and MDA-MB-435 did not have growth suppression by LGD1069 at 10 uM. LG100268 did affect little the cell growth in all 4 breast cancer cell lines suggesting its weak activity compare to LGD1069. We will further enlarge our investigation using three-dimensional (3D) epithelial culture systems, resembling their in vivo architecture to see whether the growth suppressive activity of rexinoids would be differently displayed between monolayer and 3D system. On the other hand, to investigate the mechanism by which LGD1069 suppresses breast tumorigenesis, we have studied the genes modulated by LGD1069 and LG100268. We treated first MDA-MB-231 with LGD1069 and LG100268 at 10 uM for 12 hrs, extracted RNA and then submitted to Affymetrix Microarray. In MDA-MB-231, we identified 333 genes up-regulated and 319 genes down-regulated by LGD1069 with changes in fold induction greater than 2 fold. We also identified 116 genes up-regulated and 431 genes down-regulated by LG100268 with changes in fold induction greater than 2 fold. According to the data, we found several interesting genes induced by LGD1069 and LG100268 in MDA-MB-231 such as several hypothetical proteins, CDC14 cell division cycle 14 homolog A (S. cerevisiae), recombination activating gene 2, tumor protein D52, MDM2, ITGA4, ADh1B, NF2 and cathepsin S (for LGD1069), several hypothetical proteins, zinc finger protein 423, cyclin-dependent kinase inhibitor 1C (p57, Kip2), eukaryotic translation initiation factor 5A, transcription factor 4, MDM2, FGF2, GNRH1, and annexin A9 (for LG100268). We will study their functions. We are also performing gene profiling for HMEC and T47D to identify the genes regulated by LGD 1069 and LG100268. This work is supported by U.S. DOD Grant (W81XWH-04-1-0505)." @default.
• W2230439594 created "2016-06-24" @default.
• W2230439594 creator A5023828287 @default.
• W2230439594 creator A5040511774 @default.
• W2230439594 date "2007-05-01" @default.
• W2230439594 modified "2023-09-24" @default.
• W2230439594 title "The effect of rexinoids (LGD1069 and LG100268) on the breast cell growth" @default.
• W2230439594 hasPublicationYear "2007" @default.
• W2230439594 type Work @default.
• W2230439594 sameAs 2230439594 @default.
• W2230439594 citedByCount "0" @default.
• W2230439594 crossrefType "journal-article" @default.
• W2230439594 hasAuthorship W2230439594A5023828287 @default.
• W2230439594 hasAuthorship W2230439594A5040511774 @default.
• W2230439594 hasConcept C121608353 @default.
• W2230439594 hasConcept C126322002 @default.
• W2230439594 hasConcept C1491633281 @default.
• W2230439594 hasConcept C190283241 @default.
• W2230439594 hasConcept C2777176818 @default.
• W2230439594 hasConcept C2779707156 @default.
• W2230439594 hasConcept C502942594 @default.
• W2230439594 hasConcept C530470458 @default.
• W2230439594 hasConcept C54355233 @default.
• W2230439594 hasConcept C55493867 @default.
• W2230439594 hasConcept C62112901 @default.
• W2230439594 hasConcept C71924100 @default.
• W2230439594 hasConcept C81885089 @default.
• W2230439594 hasConcept C86803240 @default.
• W2230439594 hasConcept C96232424 @default.
• W2230439594 hasConcept C98274493 @default.
• W2230439594 hasConceptScore W2230439594C121608353 @default.
• W2230439594 hasConceptScore W2230439594C126322002 @default.
• W2230439594 hasConceptScore W2230439594C1491633281 @default.
• W2230439594 hasConceptScore W2230439594C190283241 @default.
• W2230439594 hasConceptScore W2230439594C2777176818 @default.
• W2230439594 hasConceptScore W2230439594C2779707156 @default.
• W2230439594 hasConceptScore W2230439594C502942594 @default.
• W2230439594 hasConceptScore W2230439594C530470458 @default.
• W2230439594 hasConceptScore W2230439594C54355233 @default.
• W2230439594 hasConceptScore W2230439594C55493867 @default.
• W2230439594 hasConceptScore W2230439594C62112901 @default.
• W2230439594 hasConceptScore W2230439594C71924100 @default.
• W2230439594 hasConceptScore W2230439594C81885089 @default.
• W2230439594 hasConceptScore W2230439594C86803240 @default.
• W2230439594 hasConceptScore W2230439594C96232424 @default.
• W2230439594 hasConceptScore W2230439594C98274493 @default.
• W2230439594 hasLocation W22304395941 @default.
• W2230439594 hasOpenAccess W2230439594 @default.
• W2230439594 hasPrimaryLocation W22304395941 @default.
• W2230439594 hasRelatedWork W2001138013 @default.
• W2230439594 hasRelatedWork W2068022222 @default.
• W2230439594 hasRelatedWork W2072748318 @default.
• W2230439594 hasRelatedWork W2077926747 @default.
• W2230439594 hasRelatedWork W2095742968 @default.
• W2230439594 hasRelatedWork W2233186404 @default.
• W2230439594 hasRelatedWork W2320853862 @default.
• W2230439594 hasRelatedWork W2328345356 @default.
• W2230439594 hasRelatedWork W2332154466 @default.
• W2230439594 hasRelatedWork W2409741019 @default.
• W2230439594 hasRelatedWork W2489418792 @default.
• W2230439594 hasRelatedWork W2503413002 @default.
• W2230439594 hasRelatedWork W2560980306 @default.
• W2230439594 hasRelatedWork W2593247253 @default.
• W2230439594 hasRelatedWork W2741774147 @default.
• W2230439594 hasRelatedWork W2745323169 @default.
• W2230439594 hasRelatedWork W2791781412 @default.
• W2230439594 hasRelatedWork W2889583624 @default.
• W2230439594 hasRelatedWork W2988927692 @default.
• W2230439594 hasRelatedWork W3013998940 @default.
• W2230439594 hasVolume "67" @default.
• W2230439594 isParatext "false" @default.
• W2230439594 isRetracted "false" @default.
• W2230439594 magId "2230439594" @default.
• W2230439594 workType "article" @default.