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- W2230532016 abstract "Objective To investigate the immunoprotection of Streptococcus suis serotype 2(SS2) suilysin(SLY) and its antiserum.Methods Mice were immunized with the purified SLY recombinant protein.The antiserum against SLY was collected and used to determine the specific antibody titers and IgG isotypes.Subsequently,the active immunized mice were challenged with a lethal dose of S.suis 2.Moreover,the immunoprotection of SLY was evaluated.The antiserum against SLY was used passively to immunize the mice,which were challenged with a lethal dose of S.suis 2 after four hours.The protection provided by the antiserum against S.suis 2 was evaluated.Results The antiserum for mice immunized with recombinant protein SLY was collected.The titer reached 1∶25600,containing IgG1and IgG2a,two kinds of subtype antibodies.However,the difference between IgG1 and IgG2a was not significant.All actively immunized mice were injected with a lethal dose of S.suis 2.The survival rate of the actively immunized mice was significantly higher(60%) than that of the control group(10%)(P 0.05) 14 days after the challenge.Conclusions SLY and SLY-specific antiserum provides immunoprotection." @default.
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- W2230532016 date "2011-01-11" @default.
- W2230532016 modified "2023-09-22" @default.
- W2230532016 title "Immunoprotection from Streptococcus suis serotype 2 suilysin and its antiserum" @default.
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