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- W2232416123 abstract "To evaluate the biocide effect of quaternary ammonium chloride (QAC), a whitewater sample was taken from a fine papermachine headbox. By plate spreading method, 51 strains of facultative anaerobes were isolated morphologically. Forty-three strains among the isolates were found resistant to the biocide, quaternary ammonium chloride. Then the strains were separately transferred to basal medium and were incubated before the beginning of log phase. To identify strains with different QAC resistance, 30-120 ppm of N-Alkyl-benzyl-dimethyl ammonium chloride were added to basal medium. Biocide effect was evaluated by comparing bacterial growth, which can be monitored by 600 nm light absorbance of basal medium suspension. Among 51 strains, only 2 strains can survive QAC concentration up to 120 ppm. API 20E and 16S rRNA gene sequencing technique were applied to identify two strains with highest (120 ppm) QAC resistance. One strain (HB22) was identified as Morganella morganii. HB22 can resist QAC concentration up to 150 ppm. HB22 is Gram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole positive, H2S production negative, facultative anaerobe. HB22 has optimum growth in condition of 35℃ and pH 7.0. HB22 can catabolize only glucose and D(+)-Mannose. The other (HB45) was identified with high similarity among Pseudomonas cf. monteili or Pseudomonas mosselii or Pseudomonas putida. HB45 can resist QAC concentration up to 200 ppm. HB45 is Gram-negative rod, motile with flagella, catalase positive, oxidase negative, Indole negative, H2S production negative, aerobe. HB45 has optimum growth in condition of 30℃ and pH 7.4. HB45 can catabolize L-Arabinose, L(+)-Rhamnose, D(+)-Mannose, Glucose, Glycerol, Lactose, Maltose, Raffinose, Xylose, Cellulose and Xylan. The applicability of this work to paper industry is also discussed." @default.
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- W2232416123 date "2003-06-01" @default.
- W2232416123 modified "2023-09-27" @default.
- W2232416123 title "Characteristics of Quaternary Ammonium Resistant Bacteria from a Fine Papermachine System" @default.
- W2232416123 doi "https://doi.org/10.6542/efntu.200306_17(2).0003" @default.
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