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• W2233134558 abstract "Higher throughput methods to mount and collect data from multiple small and radiation-sensitive crystals are important to support challenging structural investigations using microfocus synchrotron beamlines. Furthermore, efficient sample-delivery methods are essential to carry out productive femtosecond crystallography experiments at X-ray free-electron laser (XFEL) sources such as the Linac Coherent Light Source (LCLS). To address these needs, a high-density sample grid useful as a scaffold for both crystal growth and diffraction data collection has been developed and utilized for efficient goniometer-based sample delivery at synchrotron and XFEL sources. A single grid contains 75 mounting ports and fits inside an SSRL cassette or uni-puck storage container. The use of grids with an SSRL cassette expands the cassette capacity up to 7200 samples. Grids may also be covered with a polymer film or sleeve for efficient room-temperature data collection from multiple samples. New automated routines have been incorporated into the Blu-Ice/DCSS experimental control system to support grids, including semi-automated grid alignment, fully automated positioning of grid ports, rastering and automated data collection. Specialized tools have been developed to support crystallization experiments on grids, including a universal adaptor, which allows grids to be filled by commercial liquid-handling robots, as well as incubation chambers, which support vapor-diffusion and lipidic cubic phase crystallization experiments. Experiments in which crystals were loaded into grids or grown on grids using liquid-handling robots and incubation chambers are described. Crystals were screened at LCLS-XPP and SSRL BL12-2 at room temperature and cryogenic temperatures." @default.
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• W2233134558 date "2016-01-01" @default.
• W2233134558 modified "2023-10-10" @default.
• W2233134558 title "High-density grids for efficient data collection from multiple crystals" @default.
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• W2233134558 doi "https://doi.org/10.1107/s2059798315020847" @default.
• W2233134558 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4756618" @default.
• W2233134558 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26894529" @default.
• W2233134558 hasPublicationYear "2016" @default.
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