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- W2233176135 abstract "Objective To purify the immunoglobulin from Ricefield eel,and to prepare the rabbit polyclonal antisera.Methods Ricefield eel immunoglobulin was purified using staphylococcal protein A affinity chromatography. The products were examined by SDS-PAGE electrophoresis.Rabbit antisera were prepared by repeatedly immunizing rabbits with purified IgM.And anti-Ricefield eel IgM antibodies in rabbit serum were detected by agarose double immunodiffusion.Western blotting was performed to assess specificity of rabbit antisera for Ricefield eel IgM.Results Immunoglobulin purified from Ricefield eel had only two bands in SDS-PAGE.The titer of rabbit antisera obtained was up to 1∶32 examined by agarose double immunodiffusion.The specific activity of polyclonal rabbit antisera against immunoglobulin of Ricefield eel was indicated with western blotting.Conclusions Ricefield eel immunoglobulin was successfully purified,and rabbit antisera was prepared.This provide a tool for developing the serological test system of Ricefield eel." @default.
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- W2233176135 date "2007-01-01" @default.
- W2233176135 modified "2023-09-26" @default.
- W2233176135 title "Purification of Serum Immunoglobulin from Ricefield Eel(Monopterus albus)and Preparation of Rabbit Polyclonal Antisera" @default.
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