FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2233706293> ?p ?o ?g. }

• W2233706293 endingPage "8" @default.
• W2233706293 startingPage "141" @default.
• W2233706293 abstract "In spermatogenesis, spermatogonial cells differentiate to the haploid gametes. It has been shown that spermatogenesis can be done at in vitro condition. In vitro spermatogenesis may provide an open window to treat male infertility.The aim of this study was to evaluate the effects of a novel scaffold containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) on the mouse spermatogonial cell line (SCL).First, TCP NPs were synthesized by reaction of calcium nitrate and diammonium phosphate at pH 13. Then, serial concentrations of TCP NPs were separately added to 500 mg/mL HSA, and incubated in the 100(o)C water for 30 min. In the next step, each scaffold was cut (2×2mm), placed into sterile well of microplate, and then incubated for 1, 2, and 3 days at 37(o)C with mouse SCL. After incubation, the cytotoxicity of the scaffolds was evaluated by different tests including 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) assay, vital staining, and cell counting. On the other hand, the release of TCP NPs and HSA from the scaffolds was measured.Based on microscopic observation, the size of cavities for all scaffolds was near 200-500 µm, and the size of TCP NPs was near 50-100 nm. All toxicity tests showed that the increase of TCP concentration in the scaffold did not affect mouse SCL. It means that the percentage of cell viability, LDH release, vital cells, and cell quantity was 85%, 105%, 90%, and 110%, respectively. But, the increase of incubation time led to increase of LDH release (up to 115%) and cell count (up to 115%). Also, little decrease of cell viability and vital cells was seen when incubation time was increased. Here, no release of TCP NPs and HSA was seen after increase of TCP concentration and incubation time.It can be concluded that the increase of TCP concentration in HSA/ TCP NPs scaffold does not lead to cytotoxicity. On the other hand, the increase of incubation time leads to increase of mouse SCL cell death. In this study, it was found that TCP NPs and HSA could not release from the scaffolds. In future, both proliferation and differentiation of mouse SCL on HSA/TCP NPs scaffold must be checked over more wide incubation times." @default.
• W2233706293 created "2016-06-24" @default.
• W2233706293 creator A5008719166 @default.
• W2233706293 creator A5014694850 @default.
• W2233706293 creator A5027913884 @default.
• W2233706293 creator A5081482999 @default.
• W2233706293 date "2015-03-01" @default.
• W2233706293 modified "2023-09-23" @default.
• W2233706293 title "The viability of mouse spermatogonial germ cells on a novel scaffold, containing human serum albumin and calcium phosphate nanoparticles." @default.
• W2233706293 cites W1534782054 @default.
• W2233706293 cites W1566614095 @default.
• W2233706293 cites W1576261436 @default.
• W2233706293 cites W195500608 @default.
• W2233706293 cites W196443461 @default.
• W2233706293 cites W1969170451 @default.
• W2233706293 cites W1969845288 @default.
• W2233706293 cites W1970138808 @default.
• W2233706293 cites W1975745397 @default.
• W2233706293 cites W1984202223 @default.
• W2233706293 cites W1985374659 @default.
• W2233706293 cites W1993037781 @default.
• W2233706293 cites W2001812565 @default.
• W2233706293 cites W2012629433 @default.
• W2233706293 cites W2014036694 @default.
• W2233706293 cites W2030233550 @default.
• W2233706293 cites W2041369934 @default.
• W2233706293 cites W2046266349 @default.
• W2233706293 cites W2048561250 @default.
• W2233706293 cites W2055472657 @default.
• W2233706293 cites W2062360366 @default.
• W2233706293 cites W2065109141 @default.
• W2233706293 cites W2066859997 @default.
• W2233706293 cites W2068511668 @default.
• W2233706293 cites W2074380747 @default.
• W2233706293 cites W2083387945 @default.
• W2233706293 cites W2088816522 @default.
• W2233706293 cites W2102188803 @default.
• W2233706293 cites W2111812837 @default.
• W2233706293 cites W2115565745 @default.
• W2233706293 cites W2154276303 @default.
• W2233706293 cites W2160260477 @default.
• W2233706293 cites W2305877956 @default.
• W2233706293 cites W2328364994 @default.
• W2233706293 cites W2332820070 @default.
• W2233706293 cites W2406235042 @default.
• W2233706293 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4426153" @default.
• W2233706293 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26000004" @default.
• W2233706293 hasPublicationYear "2015" @default.
• W2233706293 type Work @default.
• W2233706293 sameAs 2233706293 @default.
• W2233706293 citedByCount "3" @default.
• W2233706293 countsByYear W22337062932020 @default.
• W2233706293 countsByYear W22337062932021 @default.
• W2233706293 countsByYear W22337062932023 @default.
• W2233706293 crossrefType "journal-article" @default.
• W2233706293 hasAuthorship W2233706293A5008719166 @default.
• W2233706293 hasAuthorship W2233706293A5014694850 @default.
• W2233706293 hasAuthorship W2233706293A5027913884 @default.
• W2233706293 hasAuthorship W2233706293A5081482999 @default.
• W2233706293 hasConcept C109316439 @default.
• W2233706293 hasConcept C1491633281 @default.
• W2233706293 hasConcept C150903083 @default.
• W2233706293 hasConcept C153911025 @default.
• W2233706293 hasConcept C178790620 @default.
• W2233706293 hasConcept C181199279 @default.
• W2233706293 hasConcept C185592680 @default.
• W2233706293 hasConcept C202751555 @default.
• W2233706293 hasConcept C207001950 @default.
• W2233706293 hasConcept C2776125364 @default.
• W2233706293 hasConcept C2777318727 @default.
• W2233706293 hasConcept C2780783641 @default.
• W2233706293 hasConcept C519063684 @default.
• W2233706293 hasConcept C53227056 @default.
• W2233706293 hasConcept C55493867 @default.
• W2233706293 hasConcept C86803240 @default.
• W2233706293 hasConceptScore W2233706293C109316439 @default.
• W2233706293 hasConceptScore W2233706293C1491633281 @default.
• W2233706293 hasConceptScore W2233706293C150903083 @default.
• W2233706293 hasConceptScore W2233706293C153911025 @default.
• W2233706293 hasConceptScore W2233706293C178790620 @default.
• W2233706293 hasConceptScore W2233706293C181199279 @default.
• W2233706293 hasConceptScore W2233706293C185592680 @default.
• W2233706293 hasConceptScore W2233706293C202751555 @default.
• W2233706293 hasConceptScore W2233706293C207001950 @default.
• W2233706293 hasConceptScore W2233706293C2776125364 @default.
• W2233706293 hasConceptScore W2233706293C2777318727 @default.
• W2233706293 hasConceptScore W2233706293C2780783641 @default.
• W2233706293 hasConceptScore W2233706293C519063684 @default.
• W2233706293 hasConceptScore W2233706293C53227056 @default.
• W2233706293 hasConceptScore W2233706293C55493867 @default.
• W2233706293 hasConceptScore W2233706293C86803240 @default.
• W2233706293 hasIssue "3" @default.
• W2233706293 hasLocation W22337062931 @default.
• W2233706293 hasOpenAccess W2233706293 @default.
• W2233706293 hasPrimaryLocation W22337062931 @default.
• W2233706293 hasRelatedWork W1734035730 @default.
• W2233706293 hasRelatedWork W2027421675 @default.
• W2233706293 hasRelatedWork W2040315281 @default.

• next