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• W2233884098 startingPage "2347" @default.
• W2233884098 abstract "Abstract Fetal cells that circulate in maternal peripheral blood (PB) during pregnancy offer a potential source of nucleated fetal material for noninvasive prenatal diagnosis. Fluorescence-activated cell sorting was used to target two populations of fetal cells: nucleated erythroid cells (NECs; CD71/glycophorin-A+ CD45lo-int CD34−) and hematopoietic progenitor cells (CD34+ cells; CD34++ CD71/glycophorin-A− CD45int). Fetal cells were detected by fluorescence in situ hybridization (FISH) using directly conjugated chromosome X and Y probes in 65% (13 of 20) of the maternal PBs (fetal karyotype 46,XY). The frequency of fetal cells isolated from the NEC and CD34+ fractions was, respectively, 0 to 14 and 0 to 7 cells per 2 × 107 previously frozen maternal cells (≈20 mL of blood). In nonfrozen samples, the yield and recovery of fetal cells was moderately improved. Culturing the CD34+ sorted fractions in serum-free media with cytokines improved the quality of the FISH preparations and resulted in a slight expansion in detectable fetal cells. The frequency of fetal cells isolated from cultured CD34+ fractions was 0 to 35 and 0 to 93 cells per 2 × 107 previously frozen and nonfrozen maternal PB cells, respectively. These results document the isolation, characterization, and enumeration of fetal cells from the maternal periphery that appear to be present in most, but not all, samples analyzed." @default.
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• W2233884098 date "1997-04-01" @default.
• W2233884098 modified "2023-10-11" @default.
• W2233884098 title "Frequency of Fetal Cells in Sorted Subpopulations of Nucleated Erythroid and CD34+ Hematopoietic Progenitor Cells From Maternal Peripheral Blood" @default.
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• W2233884098 doi "https://doi.org/10.1182/blood.v89.7.2347" @default.
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