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- W2233931507 endingPage "46" @default.
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- W2233931507 abstract "This chapter reviews the methods that have been successfully used to date for the identification of RNA modification and editing enzymes. The development of recombinant DNA and RNA techniques and techniques for chemical synthesis of DNA and RNA offers adequate alternatives for obtaining synthetic or semisynthetic unmodified or partially modified RNA substrates suitable for analyzing enzymatic posttranscriptional RNA modifications both in vivo and in vitro. Stepwise automated chemical synthesis of DNA oligonucleotides is widely used for producing starting material for gene construction, primers for PCR, DNA splints, or DNA probes for hybridization and deoxyribozymes. Two distinct approaches are generally used to gain the information on the precise location of modification or editing sites in RNA molecules. The first is based on direct sequencing of pure RNA and is applied mostly for the analysis of rather short RNA molecules, while the other is based on the use of reverse transcription with unfractionated RNA mixtures and specific oligonucleotide primers. Microinjection of antisense RNA, with the aim of blocking translation of selected mRNAs in the Xenopus laevis oocyte or fertilized frog eggs, revealed the existence of what was first identified as an unwinding RNA protein. With the recent access to complete genome sequences, it has become possible to search for homologous genes in the different genomes, to clone them into suitable vectors after amplification by PCR, and to express them in appropriate host cells." @default.
- W2233931507 created "2016-06-24" @default.
- W2233931507 creator A5073956638 @default.
- W2233931507 creator A5086950475 @default.
- W2233931507 creator A5089150142 @default.
- W2233931507 date "2014-04-30" @default.
- W2233931507 modified "2023-10-11" @default.
- W2233931507 title "RNA-Modifying and RNA-Editing Enzymes: Methods for Their Identification" @default.
- W2233931507 cites W1000084 @default.
- W2233931507 cites W101969071 @default.
- W2233931507 cites W1039907071 @default.
- W2233931507 cites W1133902369 @default.
- W2233931507 cites W1435432142 @default.
- W2233931507 cites W1464706024 @default.
- W2233931507 cites W1480395323 @default.
- W2233931507 cites W1486369891 @default.
- W2233931507 cites W1497883188 @default.
- W2233931507 cites W1498439983 @default.
- W2233931507 cites W1499048201 @default.
- W2233931507 cites W1500750765 @default.
- W2233931507 cites W1517480469 @default.
- W2233931507 cites W1562507949 @default.
- W2233931507 cites W1603778040 @default.
- W2233931507 cites W1619003858 @default.
- W2233931507 cites W1628774407 @default.
- W2233931507 cites W1659527116 @default.
- W2233931507 cites W1776186831 @default.
- W2233931507 cites W1820306814 @default.
- W2233931507 cites W190429563 @default.
- W2233931507 cites W1963849742 @default.
- W2233931507 cites W1964289666 @default.
- W2233931507 cites W1966544810 @default.
- W2233931507 cites W1969096185 @default.
- W2233931507 cites W196924667 @default.
- W2233931507 cites W1970667469 @default.
- W2233931507 cites W1971314065 @default.
- W2233931507 cites W1971360355 @default.
- W2233931507 cites W1973437746 @default.
- W2233931507 cites W1974886922 @default.
- W2233931507 cites W1975412286 @default.
- W2233931507 cites W1977709085 @default.
- W2233931507 cites W1978422831 @default.
- W2233931507 cites W1979130028 @default.
- W2233931507 cites W1979442620 @default.
- W2233931507 cites W1979577604 @default.
- W2233931507 cites W1980412141 @default.
- W2233931507 cites W1982947652 @default.
- W2233931507 cites W1985012667 @default.
- W2233931507 cites W1985217440 @default.
- W2233931507 cites W1985443292 @default.
- W2233931507 cites W1987200697 @default.
- W2233931507 cites W1987827036 @default.
- W2233931507 cites W1988085321 @default.
- W2233931507 cites W1988556567 @default.
- W2233931507 cites W1993154139 @default.
- W2233931507 cites W1993480746 @default.
- W2233931507 cites W1993727713 @default.
- W2233931507 cites W1994926187 @default.
- W2233931507 cites W1994938472 @default.
- W2233931507 cites W1995467090 @default.
- W2233931507 cites W1997774432 @default.
- W2233931507 cites W1999690794 @default.
- W2233931507 cites W2001218072 @default.
- W2233931507 cites W2001870142 @default.
- W2233931507 cites W2002151098 @default.
- W2233931507 cites W2002404401 @default.
- W2233931507 cites W2004964102 @default.
- W2233931507 cites W2005637337 @default.
- W2233931507 cites W2006144062 @default.
- W2233931507 cites W2006762876 @default.
- W2233931507 cites W2006871585 @default.
- W2233931507 cites W2008149648 @default.
- W2233931507 cites W2008450625 @default.
- W2233931507 cites W2010273344 @default.
- W2233931507 cites W2010299178 @default.
- W2233931507 cites W2010899064 @default.
- W2233931507 cites W2011057627 @default.
- W2233931507 cites W2011431590 @default.
- W2233931507 cites W2015109869 @default.
- W2233931507 cites W2017598424 @default.
- W2233931507 cites W2017826761 @default.
- W2233931507 cites W2020747171 @default.
- W2233931507 cites W2020804327 @default.
- W2233931507 cites W2024412145 @default.
- W2233931507 cites W2027190461 @default.
- W2233931507 cites W2027650483 @default.
- W2233931507 cites W2028625367 @default.
- W2233931507 cites W2028819432 @default.
- W2233931507 cites W2029130204 @default.
- W2233931507 cites W2030302639 @default.
- W2233931507 cites W2030813091 @default.
- W2233931507 cites W2031615793 @default.
- W2233931507 cites W2037691907 @default.
- W2233931507 cites W2038794735 @default.
- W2233931507 cites W2038989483 @default.
- W2233931507 cites W2040174498 @default.
- W2233931507 cites W2044410490 @default.
- W2233931507 cites W2048858950 @default.