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- W2234993068 abstract "The aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects onrumen anaerobic fungal population in lactation Iranian of Holstein cows. From d 5 to 56 postpartum, cows were fed diets thatwere isoenergetic containing soybean meal (SBM; n = 3) or canola meal (CM; n = 3). Cows were housed in tie stalls and fedTMR two times a day. Competitive PCR technique was used to evaluate quantitative difference of anaerobic fungalpopulation within the rumen under the dietary groups. Universal primers were used to amplify a specific region of the rRNAlocus from anaerobic rumen fungi. Standard control DNA was constructed for use in the competitive PCR and was shown toamplify under the same reaction condition and with the same amplification efficiency as the target DNA. The relativeintensities of PCR products were used to compare variety of fungal population under fed treatments. The signal intensity wasquantified by ImageJ 1.29x and expressed in arbitrary units. The data was analyzed using the GLM procedure of SAS for acompletely randomized design. The rumen fungal population was not impacted by diet but numerically was decreased in theCM compare with the SBM diet (34.79 and 35.12 ± 1.87 arbitrary units, respectively). The results of this study demonstratedthat substituting soybean meal with canola meal in the early lactation cows had no apparent effect on the fungal population.." @default.
- W2234993068 created "2016-06-24" @default.
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- W2234993068 date "2009-05-22" @default.
- W2234993068 modified "2023-10-18" @default.
- W2234993068 title "بررسی تاثیر جایگزینی کنجاله کلزا با کنجاله سویا بر جمعیت قارچ های بی هوازی شکمبه با استفاده از روش رقابتی PCR" @default.
- W2234993068 doi "https://doi.org/10.22067/ijasr.v1i2.1981" @default.
- W2234993068 hasPublicationYear "2009" @default.
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