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- W2235166629 abstract "Introduction: The use of antibiotics – including the overand misuse – in human and veterinary practices selected for resistant pathogens led to their emergence and dissemination along with the transmission of resistant bacteria [1]. Due to this problem, there is a need to investigate new strategies to reverse this tendency in order to achieve the appropriate and effective treatment against infections. Aims: The present work aimed to evaluate the antibacterial activity of an aqueous extract prepared from Euterpe Oleracea Mart. (acai) seeds (originated from Brazil [2]), against clinical isolates from “Centro Hospitalar de Tras-os-Montes e Alto DouroUnidade de Vila Real”, with high resistance profiles to different antibiotics. Material and Methods: The minimal inhibitory concentrations (MIC) of the growth of Gram-positive and Gram-negative bacteria were determined by the colorimetric assay based on the use of p-iodonitrotetrazolium chloride (INT) dye, after application of the microdilution method. Results and discussion: The acai aqueous extract showed antimicrobial activity against all the tested Gram-positive bacteria. The lowest MICs (0.25 mg/ml) were obtained against Methicillin Susceptible Staphylococcus aureus (MSSA) and Methicillin Resistant S. aureus (MRSA), followed by the MIC 0.5 mg/ml correspondent to Streptococcus agalactiae and Enterococcus faecalis. Regarding the inhibition of the growth of Gram-negative bacteria, the extract was active against Morganella morganii, Pseudomonas aeruginosa and Acinetobacter baumannii with MICs 1 mg/ml e 2 mg/ml, respectively. The results were better for Gram-positive bacteria in comparison with Gram-negative bacteria. Conclusion: However, even at low concentration (2 mg/ml) the results are very promising for Gram-negative bacteria with high resistance profiles and commonly related with health care-associated infections, namely Pseudomonas aeruginosa and Acinetobacter baumannii. Key-words: Clinical isolates; multiresistance; acai seeds; antibacterial activity References [1] Marshall, B.M., Levy, S.B., 2011. Food animals and antimicrobials: impacts on human health. Clin. Microbiol. Rev. 24, 718–733. [2] Barros, L., Calhelha, R.C., Queiroz, M.J.R.P., Santos-Buelga, C., Santos, E.A., Regis, W.C.B., Ferreira, I.C.F.R. 2015. The powerful in vitro bioactivity of Euterpe oleracea Mart. seeds and related phenolic compounds. Ind. Crop. Prod. 76, 318–322. CERTIF CADO DE PARTI C PACAO A Escola Superior de Tecnologia da Saude do Porto (ESTSP) IPP certifica que Maria Alves apresentou a Comunicacao Oral, inserida na Sessao de Comunicacoes Orais Tema Livre/ intitulada Antibacteria! activity of Euterpe oieracea Mart. seeds against clinicai isolates da autoria de Maria Alves e (CO-AUTORES) Helena Pimentel/ Isabel Ferreira, Wiliam Regis, Lilia Baia, Rafaela Soares e Claudia Santos no II Congresso Internacional de Saude Gaia-Porto Do Diagnostico a Intervencao que decorreu no ISEP (Instituto Superior de Engenharia do Porto) no Porto, nos dias 19, 20-e 21 de" @default.
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- W2235166629 date "2015-01-01" @default.
- W2235166629 modified "2023-10-18" @default.
- W2235166629 title "Antibacterial activity of Euterpe oieracea Mart. seeds against clinical isolates" @default.
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