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• W2235247229 endingPage "3427" @default.
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• W2235247229 abstract "ABSTRACT Adenovirus (Ad) replication compartments (RC) are nuclear microenvironments where the viral genome is replicated and a coordinated program of late gene expression is established. These virus-induced nuclear sites seem to behave as central hubs for the regulation of virus-host cell interactions, since proteins that promote efficient viral replication as well as factors that participate in the antiviral response are coopted and concentrated there. To gain further insight into the activities of viral RC, here we report, for the first time, the morphology, composition, and activities of RC isolated from Ad-infected cells. Morphological analyses of isolated RC particles by superresolution microscopy showed that they were indistinguishable from RC within infected cells and that they displayed a dynamic compartmentalization. Furthermore, the RC-containing fractions (RCf) proved to be functional, as they directed de novo synthesis of viral DNA and RNA as well as RNA splicing, activities that are associated with RC in vivo . A detailed analysis of the production of viral late mRNA from RCf at different times postinfection revealed that viral mRNA splicing occurs in RC and that the synthesis, posttranscriptional processing, and release from RC to the nucleoplasm of individual viral late transcripts are spatiotemporally separate events. The results presented here demonstrate that RCf are a powerful system for detailed study into RC structure, composition, and activities and, as a result, the determination of the molecular mechanisms that induce the formation of these viral sites of adenoviruses and other nuclear-replicating viruses. IMPORTANCE RC may represent molecular hubs where many aspects of virus-host cell interaction are controlled. Here, we show by superresolution microscopy that RCf have morphologies similar to those of RC within Ad-infected cells and that they appear to be compartmentalized, as nucleolin and DBP display different localization in the periphery of these viral sites. RCf proved to be functional, as they direct de novo synthesis of viral DNA and mRNA, allowing the detailed study of the regulation of viral genome replication and expression. Furthermore, we show that the synthesis and splicing of individual viral late mRNA occurs in RC and that they are subject to different temporal patterns of regulation, from their synthesis to their splicing and release from RC to the nucleoplasm. Hence, RCf represent a novel system to study molecular mechanisms that are orchestrated in viral RC to take control of the infected cell and promote an efficient viral replication cycle." @default.
• W2235247229 created "2016-06-24" @default.
• W2235247229 creator A5000413759 @default.
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• W2235247229 creator A5055700105 @default.
• W2235247229 creator A5059530844 @default.
• W2235247229 creator A5073849274 @default.
• W2235247229 date "2016-04-01" @default.
• W2235247229 modified "2023-10-15" @default.
• W2235247229 title "Morphological, Biochemical, and Functional Study of Viral Replication Compartments Isolated from Adenovirus-Infected Cells" @default.
• W2235247229 cites W135925168 @default.
• W2235247229 cites W1511378991 @default.
• W2235247229 cites W1576789299 @default.
• W2235247229 cites W1584657520 @default.
• W2235247229 cites W161101904 @default.
• W2235247229 cites W1665409553 @default.
• W2235247229 cites W1766360662 @default.
• W2235247229 cites W1775164461 @default.
• W2235247229 cites W1789989063 @default.
• W2235247229 cites W1834610545 @default.
• W2235247229 cites W1922329782 @default.
• W2235247229 cites W1934900858 @default.
• W2235247229 cites W1964629933 @default.
• W2235247229 cites W1964644257 @default.
• W2235247229 cites W1972440264 @default.
• W2235247229 cites W1978048533 @default.
• W2235247229 cites W1979520474 @default.
• W2235247229 cites W1979772572 @default.
• W2235247229 cites W1982772527 @default.
• W2235247229 cites W1987652798 @default.
• W2235247229 cites W1989061838 @default.
• W2235247229 cites W1990486157 @default.
• W2235247229 cites W1990580642 @default.
• W2235247229 cites W1991823556 @default.
• W2235247229 cites W1992200158 @default.
• W2235247229 cites W1992913991 @default.
• W2235247229 cites W1993072500 @default.
• W2235247229 cites W1993986305 @default.
• W2235247229 cites W1999064296 @default.
• W2235247229 cites W2001783764 @default.
• W2235247229 cites W2004624749 @default.
• W2235247229 cites W2005026309 @default.
• W2235247229 cites W2005490545 @default.
• W2235247229 cites W2008728434 @default.
• W2235247229 cites W2022077328 @default.
• W2235247229 cites W2024026665 @default.
• W2235247229 cites W2035078024 @default.
• W2235247229 cites W2035755400 @default.
• W2235247229 cites W2038454995 @default.
• W2235247229 cites W2039809624 @default.
• W2235247229 cites W2042281993 @default.
• W2235247229 cites W2043368790 @default.
• W2235247229 cites W2045647047 @default.
• W2235247229 cites W2047095396 @default.
• W2235247229 cites W2051537046 @default.
• W2235247229 cites W2053516485 @default.
• W2235247229 cites W2055515767 @default.
• W2235247229 cites W2056438653 @default.
• W2235247229 cites W2059763393 @default.
• W2235247229 cites W2064842780 @default.
• W2235247229 cites W2064982582 @default.
• W2235247229 cites W2065262709 @default.
• W2235247229 cites W2067047912 @default.
• W2235247229 cites W2067131377 @default.
• W2235247229 cites W2067517387 @default.
• W2235247229 cites W2069738082 @default.
• W2235247229 cites W2069816698 @default.
• W2235247229 cites W2071142061 @default.
• W2235247229 cites W2071755568 @default.
• W2235247229 cites W2072274257 @default.
• W2235247229 cites W2073097074 @default.
• W2235247229 cites W2073600895 @default.
• W2235247229 cites W2079233637 @default.
• W2235247229 cites W2079457598 @default.
• W2235247229 cites W2080589015 @default.
• W2235247229 cites W2080657998 @default.
• W2235247229 cites W2084061272 @default.
• W2235247229 cites W2085388950 @default.
• W2235247229 cites W2089993413 @default.
• W2235247229 cites W2092333889 @default.
• W2235247229 cites W2093373040 @default.
• W2235247229 cites W2093646238 @default.
• W2235247229 cites W2099540110 @default.
• W2235247229 cites W2102938475 @default.
• W2235247229 cites W2105075502 @default.
• W2235247229 cites W2105576556 @default.
• W2235247229 cites W2108704467 @default.
• W2235247229 cites W2116759890 @default.
• W2235247229 cites W2116951270 @default.
• W2235247229 cites W2121023964 @default.
• W2235247229 cites W2121868771 @default.
• W2235247229 cites W2123659736 @default.
• W2235247229 cites W2127193558 @default.
• W2235247229 cites W2127989699 @default.
• W2235247229 cites W2129464214 @default.

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