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- W2235525850 abstract "The chapter describes method for the purification of large quantities of unfractionated sRNA from yeast. The product is essentially free from contaminants and is very stable. After an initial ethanol precipitation of the crude sRNA, the preparation is extracted with phenol to remove traces of nuclease activity and denatured proteins. Ammonium sulfate is added to precipitate high molecular weight contaminants, leaving sRNA in solution; the sRNA is recovered by precipitation with a higher concentration of ammonium sulfate. Dialysis against ethylenediaminetetraacetic acid (EDTA) removes polyvalent cations, and prolonged dialysis against sodium chloride and water gives a preparation free from contaminating small molecules. The material is finally freeze dried and stored as a solid." @default.
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- W2235525850 date "1967-01-01" @default.
- W2235525850 modified "2023-10-13" @default.
- W2235525850 title "[79] Preparation of highly purified sRNA from yeast" @default.
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- W2235525850 doi "https://doi.org/10.1016/s0076-6879(67)12091-0" @default.
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