FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2236019674> ?p ?o ?g. }

• W2236019674 abstract "Author(s): Mercurio, Kevin | Advisor(s): Leppert, Valerie | Abstract: Cell culture substrates for contact guidance studies can be developed through polymer processing to produce various topographic dimensions. Surface topography has a large influence on cell fate and can ultimately be used to help understand and direct cell behaviors. Fabrication techniques for nano- and micro-patterning are typically costly and require large, laboratory-grade equipment. However, in the past decade, researchers have discovered shrink-based techniques to produce nano-scale topography at a fraction of the cost. The fabrication methods are simple, fast, and cost effective, when compared to lithography-based patterning methods. 2D topography is developed using a lab-on-a-chip approach, in which pre-strained polystyrene sheets are coated with metal, constrained from the edges, and heat-treated to develop 320nm and 510nm surface wrinkles. An increase in metal coating thickness from 15nm to 60nm resulted in an increase in wrinkle width and height. To build upon this phenomenon, the polystyrene sheets were further manipulated to generate 3D topography. Acetone solvent was used to craze the polymer sheet and develop surface topography on the micro-scale. Depending on sheet orientation and treatment procedure, wrinkles, channels, and voids ranging in width from 1 μm to 210 μm can be produced. These patterned sheets were then used to mold polydimethylsiloxane (PDMS) cell culture platforms. Biological analysis showed good cellular adhesion of human umbilical vein endothelial cells (HUVECs) on the structures surface. Cellular orientation and actin fiber elongation were analyzed on flat controls, nano-scale wrinkles, crazed topography, and shrunk-crazed PDMS substrates after 24 hours of culture using optical microscopy. HUVECs on the acetone crazed topographies extended the actin filaments roughly 23 μm in length as compared to the flat control having an average length of 51 μm. The shrunk-crazed topography produced better cellular alignment than the crazed topography. Longitudinal and transverse shrunk samples produced cell alignment of about 10 ± 9°and 8 ± 7° from the underlying pattern direction, respectively. In addition to cell analysis on open faced surfaces, cells were also cultured in 3D microchanneled structures to demonstrate the guidance of cells in a dynamically rotating environment. Cells were loaded into PDMS tubular networks and rotated at approximately 1 rotation per hour. As a result, human umbilical vein endothelial cells (HUVECs) and rat aortic smooth muscle cells (RAOSMCs) were able to adhere to the inner surfaces of the channel and confluent layers were formed circumferentially. The use and application for the structures and devices discussed herein are analyzed and evaluated in three separate projects for their potential in bioengineering and tissue engineering applications." @default.
• W2236019674 created "2016-06-24" @default.
• W2236019674 creator A5068164092 @default.
• W2236019674 date "2013-01-01" @default.
• W2236019674 modified "2023-09-23" @default.
• W2236019674 title "Guiding Cell Behavior in 2D, 3D, and Dynamic Environments" @default.
• W2236019674 hasPublicationYear "2013" @default.
• W2236019674 type Work @default.
• W2236019674 sameAs 2236019674 @default.
• W2236019674 citedByCount "0" @default.
• W2236019674 crossrefType "journal-article" @default.
• W2236019674 hasAuthorship W2236019674A5068164092 @default.
• W2236019674 hasConcept C118524077 @default.
• W2236019674 hasConcept C136525101 @default.
• W2236019674 hasConcept C142724271 @default.
• W2236019674 hasConcept C159985019 @default.
• W2236019674 hasConcept C171250308 @default.
• W2236019674 hasConcept C192562407 @default.
• W2236019674 hasConcept C204223013 @default.
• W2236019674 hasConcept C204787440 @default.
• W2236019674 hasConcept C2777872592 @default.
• W2236019674 hasConcept C2777922577 @default.
• W2236019674 hasConcept C2779849746 @default.
• W2236019674 hasConcept C2781448156 @default.
• W2236019674 hasConcept C49040817 @default.
• W2236019674 hasConcept C521977710 @default.
• W2236019674 hasConcept C58943365 @default.
• W2236019674 hasConcept C71924100 @default.
• W2236019674 hasConcept C8673954 @default.
• W2236019674 hasConceptScore W2236019674C118524077 @default.
• W2236019674 hasConceptScore W2236019674C136525101 @default.
• W2236019674 hasConceptScore W2236019674C142724271 @default.
• W2236019674 hasConceptScore W2236019674C159985019 @default.
• W2236019674 hasConceptScore W2236019674C171250308 @default.
• W2236019674 hasConceptScore W2236019674C192562407 @default.
• W2236019674 hasConceptScore W2236019674C204223013 @default.
• W2236019674 hasConceptScore W2236019674C204787440 @default.
• W2236019674 hasConceptScore W2236019674C2777872592 @default.
• W2236019674 hasConceptScore W2236019674C2777922577 @default.
• W2236019674 hasConceptScore W2236019674C2779849746 @default.
• W2236019674 hasConceptScore W2236019674C2781448156 @default.
• W2236019674 hasConceptScore W2236019674C49040817 @default.
• W2236019674 hasConceptScore W2236019674C521977710 @default.
• W2236019674 hasConceptScore W2236019674C58943365 @default.
• W2236019674 hasConceptScore W2236019674C71924100 @default.
• W2236019674 hasConceptScore W2236019674C8673954 @default.
• W2236019674 hasLocation W22360196741 @default.
• W2236019674 hasOpenAccess W2236019674 @default.
• W2236019674 hasPrimaryLocation W22360196741 @default.
• W2236019674 hasRelatedWork W1951289170 @default.
• W2236019674 hasRelatedWork W1975065120 @default.
• W2236019674 hasRelatedWork W2018075471 @default.
• W2236019674 hasRelatedWork W2030231709 @default.
• W2236019674 hasRelatedWork W2046411392 @default.
• W2236019674 hasRelatedWork W2049542013 @default.
• W2236019674 hasRelatedWork W2049878449 @default.
• W2236019674 hasRelatedWork W2066036537 @default.
• W2236019674 hasRelatedWork W2068798459 @default.
• W2236019674 hasRelatedWork W2081471470 @default.
• W2236019674 hasRelatedWork W2105868784 @default.
• W2236019674 hasRelatedWork W2162452588 @default.
• W2236019674 hasRelatedWork W2164828135 @default.
• W2236019674 hasRelatedWork W2241671801 @default.
• W2236019674 hasRelatedWork W2579839683 @default.
• W2236019674 hasRelatedWork W2736853916 @default.
• W2236019674 hasRelatedWork W2896328651 @default.
• W2236019674 hasRelatedWork W58032669 @default.
• W2236019674 hasRelatedWork W60518284 @default.
• W2236019674 hasRelatedWork W2046650966 @default.
• W2236019674 isParatext "false" @default.
• W2236019674 isRetracted "false" @default.
• W2236019674 magId "2236019674" @default.
• W2236019674 workType "article" @default.