FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2236138476> ?p ?o ?g. }

• W2236138476 endingPage "1066" @default.
• W2236138476 startingPage "1055" @default.
• W2236138476 abstract "1. Lowered osmolality promotes epileptiform activity both clinically and in the hippocampal slice preparation, but it is unclear how neurons are excited. We studied the effects of altered osmolality on the electrophysiological properties of CA1 pyramidal cells in hippocampal slices by the use of field and intracellular recordings. The excitability of these neurons under various osmotic conditions was gauged by population spike (PS) amplitude, single cell properties, and evoked synaptic input. 2. The orthodromic PS recorded in stratum pyramidale and the field excitatory postsynaptic potential (EPSP) in stratum radiatum were inversely proportional in amplitude to the artificial cerebrospinal fluid (ACSF) osmolality over a range of +/- 80 milliosmoles/kgH2O (mosM). The effect was osmotic because changes occurred within the time frame expected for cellular expansion or shrinkage and because permeable substances such as dimethyl sulfoxide or glycerol were without effect. Dilutional changes in ACSF constituents were experimentally ruled out as promoting excitability. 3. To test whether the field data resulted from a change in single-cell excitability, CA1 cells were intracellularly recorded during exposure to +/- 40 mosM ACSF over 15 min. There was no consistent effect upon CA1 resting potential, cell input resistance, or action potential threshold. 4. Osmotic alteration of orthodromic and antidromic field potentials might involve a change in axonal excitability. However, the evoked afferent volley recorded in CA1 stratum pyramidale or radiatum, which represents the compound action potential (CAP) generated in presynaptic axons, remained osmotically unresponsive with regard to amplitude, duration, or latency. This was also characteristic of CAPs evoked in isolated sciatic and vagus nerve preparations exposed to +/- 80 mosM. Therefore axonal excitability and associated extracellular current flow generated periaxonally are not significantly affected by osmotic shifts. 5. The osmotic effect on field potential amplitudes appeared to be independent of synaptic transmission because the inverse relationship with osmolality held for the antidromically evoked PS. Moreover, as recorded with respect to ground, the intracellular EPSP-inhibitory postsynaptic potential (IPSP) sequence (evoked from CA3 stratum radiatum) was not altered by osmolality. 6. The PS could occasionally be recorded intracellularly as a brief negativity interrupting the evoked EPSP. In hyposmotic ACSF, the amplitude increased and action potentials arose from the trough of the negativity as expected for a field effect. This is presumably the result of enhanced intracellular channeling of current caused by the increased extracellular resistance that accompanies cellular swelling.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2236138476 created "2016-06-24" @default.
• W2236138476 creator A5002038049 @default.
• W2236138476 creator A5063659669 @default.
• W2236138476 creator A5087468075 @default.
• W2236138476 date "1991-05-01" @default.
• W2236138476 modified "2023-09-27" @default.
• W2236138476 title "Osmotic effects on the CA1 neuronal population in hippocampal slices with special reference to glucose" @default.
• W2236138476 doi "https://doi.org/10.1152/jn.1991.65.5.1055" @default.
• W2236138476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1651372" @default.
• W2236138476 hasPublicationYear "1991" @default.
• W2236138476 type Work @default.
• W2236138476 sameAs 2236138476 @default.
• W2236138476 citedByCount "56" @default.
• W2236138476 countsByYear W22361384762012 @default.
• W2236138476 countsByYear W22361384762013 @default.
• W2236138476 countsByYear W22361384762014 @default.
• W2236138476 countsByYear W22361384762015 @default.
• W2236138476 countsByYear W22361384762016 @default.
• W2236138476 countsByYear W22361384762017 @default.
• W2236138476 countsByYear W22361384762019 @default.
• W2236138476 countsByYear W22361384762021 @default.
• W2236138476 countsByYear W22361384762022 @default.
• W2236138476 crossrefType "journal-article" @default.
• W2236138476 hasAuthorship W2236138476A5002038049 @default.
• W2236138476 hasAuthorship W2236138476A5063659669 @default.
• W2236138476 hasAuthorship W2236138476A5087468075 @default.
• W2236138476 hasConcept C112592302 @default.
• W2236138476 hasConcept C12554922 @default.
• W2236138476 hasConcept C129900496 @default.
• W2236138476 hasConcept C148762608 @default.
• W2236138476 hasConcept C169760540 @default.
• W2236138476 hasConcept C17077164 @default.
• W2236138476 hasConcept C181911157 @default.
• W2236138476 hasConcept C185263204 @default.
• W2236138476 hasConcept C185592680 @default.
• W2236138476 hasConcept C2778437258 @default.
• W2236138476 hasConcept C2779277159 @default.
• W2236138476 hasConcept C2908647359 @default.
• W2236138476 hasConcept C55493867 @default.
• W2236138476 hasConcept C70471684 @default.
• W2236138476 hasConcept C71924100 @default.
• W2236138476 hasConcept C86803240 @default.
• W2236138476 hasConcept C96376961 @default.
• W2236138476 hasConcept C99454951 @default.
• W2236138476 hasConceptScore W2236138476C112592302 @default.
• W2236138476 hasConceptScore W2236138476C12554922 @default.
• W2236138476 hasConceptScore W2236138476C129900496 @default.
• W2236138476 hasConceptScore W2236138476C148762608 @default.
• W2236138476 hasConceptScore W2236138476C169760540 @default.
• W2236138476 hasConceptScore W2236138476C17077164 @default.
• W2236138476 hasConceptScore W2236138476C181911157 @default.
• W2236138476 hasConceptScore W2236138476C185263204 @default.
• W2236138476 hasConceptScore W2236138476C185592680 @default.
• W2236138476 hasConceptScore W2236138476C2778437258 @default.
• W2236138476 hasConceptScore W2236138476C2779277159 @default.
• W2236138476 hasConceptScore W2236138476C2908647359 @default.
• W2236138476 hasConceptScore W2236138476C55493867 @default.
• W2236138476 hasConceptScore W2236138476C70471684 @default.
• W2236138476 hasConceptScore W2236138476C71924100 @default.
• W2236138476 hasConceptScore W2236138476C86803240 @default.
• W2236138476 hasConceptScore W2236138476C96376961 @default.
• W2236138476 hasConceptScore W2236138476C99454951 @default.
• W2236138476 hasIssue "5" @default.
• W2236138476 hasLocation W22361384761 @default.
• W2236138476 hasLocation W22361384762 @default.
• W2236138476 hasOpenAccess W2236138476 @default.
• W2236138476 hasPrimaryLocation W22361384761 @default.
• W2236138476 hasRelatedWork W1972517461 @default.
• W2236138476 hasRelatedWork W1974654402 @default.
• W2236138476 hasRelatedWork W2013160497 @default.
• W2236138476 hasRelatedWork W2027265904 @default.
• W2236138476 hasRelatedWork W2041440251 @default.
• W2236138476 hasRelatedWork W2062666447 @default.
• W2236138476 hasRelatedWork W2087123550 @default.
• W2236138476 hasRelatedWork W2236138476 @default.
• W2236138476 hasRelatedWork W271802534 @default.
• W2236138476 hasRelatedWork W2293406847 @default.
• W2236138476 hasVolume "65" @default.
• W2236138476 isParatext "false" @default.
• W2236138476 isRetracted "false" @default.
• W2236138476 magId "2236138476" @default.
• W2236138476 workType "article" @default.