Matches in SemOpenAlex for { <https://semopenalex.org/work/W2236183713> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2236183713 abstract "Wnt5a is involved in the activation of human hepatic stellate cells (HSC) and related with the occurrence of liver fibrosis. As the function and mechanism that Wnt5a mediates HSC activation remains unclear, we sought to investigate them.Wnt5a levels were determined in the HSC cell line LX-2 after lipopolysaccharide (LPS) and TNF-α stimulation. HSC cells showing stable and efficient overexpression or featuring knockdown of Wnt5a were constructed by a lentivirus system. Regulation of cytokine and collagen expressions were confirmed by quantitative PCR or ELISA in stable LX-2 cell lines showing Wnt5a overexpression or knockdown. Proliferation was determined by 5-ethynyl-2'-deoxyuridine labeling. Relevant signaling pathways were identified using specific protein antibodies.LPS and TNF-α induced Wnt5a expression in LX-2 cells. Compared with control cells, an increase in IL-10, IL-6, COL1, and COL3 secretion in a stable LX-2 cell line showing Wnt5a overexpression was observed. Knockdown of Wnt5a obviously reduced the production of IL-1β, IL-6, COL1, and COL3. Wnt5a overexpression promoted LX-2 proliferation, while Wnt5a knockdown dramatically inhibited cell proliferation. Compared with the effects of Wnt5a knockdown cells, Wnt5a-overexpressing cells triggered the phosphorylation of c-Jun N-terminal kinase (JNK) and β-catenin, which leads to β-catenin degradation and inactivates the canonical Wnt/β-catenin pathway.Wnt5a regulates inflammatory cytokine and collagen production and cell proliferation, which is independent of the canonical Wnt signaling pathway. The results reveal a new signaling mechanism in HSC activation and could provide a new strategy for hepatic fibrosis treatment." @default.
- W2236183713 created "2016-06-24" @default.
- W2236183713 creator A5029237663 @default.
- W2236183713 creator A5029791678 @default.
- W2236183713 creator A5035740553 @default.
- W2236183713 creator A5060970627 @default.
- W2236183713 creator A5079780089 @default.
- W2236183713 creator A5080280424 @default.
- W2236183713 creator A5083577512 @default.
- W2236183713 date "2015-01-01" @default.
- W2236183713 modified "2023-10-14" @default.
- W2236183713 title "Wnt5a Promotes Cytokines Production and Cell Proliferation in Human Hepatic Stellate Cells Independent of Canonical Wnt Pathway" @default.
- W2236183713 doi "https://doi.org/10.7754/clin.lab.2014.141127" @default.
- W2236183713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26118187" @default.
- W2236183713 hasPublicationYear "2015" @default.
- W2236183713 type Work @default.
- W2236183713 sameAs 2236183713 @default.
- W2236183713 citedByCount "14" @default.
- W2236183713 countsByYear W22361837132015 @default.
- W2236183713 countsByYear W22361837132016 @default.
- W2236183713 countsByYear W22361837132017 @default.
- W2236183713 countsByYear W22361837132018 @default.
- W2236183713 countsByYear W22361837132019 @default.
- W2236183713 countsByYear W22361837132020 @default.
- W2236183713 countsByYear W22361837132021 @default.
- W2236183713 countsByYear W22361837132022 @default.
- W2236183713 crossrefType "journal-article" @default.
- W2236183713 hasAuthorship W2236183713A5029237663 @default.
- W2236183713 hasAuthorship W2236183713A5029791678 @default.
- W2236183713 hasAuthorship W2236183713A5035740553 @default.
- W2236183713 hasAuthorship W2236183713A5060970627 @default.
- W2236183713 hasAuthorship W2236183713A5079780089 @default.
- W2236183713 hasAuthorship W2236183713A5080280424 @default.
- W2236183713 hasAuthorship W2236183713A5083577512 @default.
- W2236183713 hasConcept C134018914 @default.
- W2236183713 hasConcept C137620995 @default.
- W2236183713 hasConcept C153911025 @default.
- W2236183713 hasConcept C173396325 @default.
- W2236183713 hasConcept C176891718 @default.
- W2236183713 hasConcept C185592680 @default.
- W2236183713 hasConcept C203014093 @default.
- W2236183713 hasConcept C2778690821 @default.
- W2236183713 hasConcept C2779324961 @default.
- W2236183713 hasConcept C2780176071 @default.
- W2236183713 hasConcept C54355233 @default.
- W2236183713 hasConcept C55493867 @default.
- W2236183713 hasConcept C62112901 @default.
- W2236183713 hasConcept C62478195 @default.
- W2236183713 hasConcept C81885089 @default.
- W2236183713 hasConcept C86803240 @default.
- W2236183713 hasConcept C95444343 @default.
- W2236183713 hasConceptScore W2236183713C134018914 @default.
- W2236183713 hasConceptScore W2236183713C137620995 @default.
- W2236183713 hasConceptScore W2236183713C153911025 @default.
- W2236183713 hasConceptScore W2236183713C173396325 @default.
- W2236183713 hasConceptScore W2236183713C176891718 @default.
- W2236183713 hasConceptScore W2236183713C185592680 @default.
- W2236183713 hasConceptScore W2236183713C203014093 @default.
- W2236183713 hasConceptScore W2236183713C2778690821 @default.
- W2236183713 hasConceptScore W2236183713C2779324961 @default.
- W2236183713 hasConceptScore W2236183713C2780176071 @default.
- W2236183713 hasConceptScore W2236183713C54355233 @default.
- W2236183713 hasConceptScore W2236183713C55493867 @default.
- W2236183713 hasConceptScore W2236183713C62112901 @default.
- W2236183713 hasConceptScore W2236183713C62478195 @default.
- W2236183713 hasConceptScore W2236183713C81885089 @default.
- W2236183713 hasConceptScore W2236183713C86803240 @default.
- W2236183713 hasConceptScore W2236183713C95444343 @default.
- W2236183713 hasIssue "05+06/2015" @default.
- W2236183713 hasLocation W22361837131 @default.
- W2236183713 hasLocation W22361837132 @default.
- W2236183713 hasOpenAccess W2236183713 @default.
- W2236183713 hasPrimaryLocation W22361837131 @default.
- W2236183713 hasRelatedWork W1563128033 @default.
- W2236183713 hasRelatedWork W2118807251 @default.
- W2236183713 hasRelatedWork W2236183713 @default.
- W2236183713 hasRelatedWork W2354733523 @default.
- W2236183713 hasRelatedWork W2374209378 @default.
- W2236183713 hasRelatedWork W2605260329 @default.
- W2236183713 hasRelatedWork W2751157603 @default.
- W2236183713 hasRelatedWork W2920999680 @default.
- W2236183713 hasRelatedWork W3085889838 @default.
- W2236183713 hasRelatedWork W4224326588 @default.
- W2236183713 hasVolume "61" @default.
- W2236183713 isParatext "false" @default.
- W2236183713 isRetracted "false" @default.
- W2236183713 magId "2236183713" @default.
- W2236183713 workType "article" @default.