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- W2237127110 abstract "This chapter describes the Purification procedure of nitrogenase proteins from various species of organisms including Bacillus polymyxa, Rhodospirillum rubrum, Clostridium pasteurianum, Klebsiella pneumoniae, and Azotobacter vinelandii. The condition of the purification of the nitrogenase proteins is that all steps to be performed under strictly anaerobic conditions. The nitrogenase of Bacillus polymyxa is one of the more refractory nitrogenases to purify. The instability of the enzyme preparations and the difficulty in their purification have restricted previous work to partially purified extracts, but now essentially pure protein components are obtained. Although this limits the techniques applicable to the purification of nitrogenase, the nitrogenase proteins from several organisms have been highly purified. The techniques most often used are diethylaminoethyl–cellulose (DEAE–cellulose) chromatography, protamine sulfate precipitation, gel filtration, and preparative gel electrophoresis. The nitrogenase proteins from other organisms are partially purified and characterized. Usually the Mo–Fe protein, the more stable of the two proteins, can be purified to a greater degree than the Fe protein." @default.
- W2237127110 created "2016-06-24" @default.
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- W2237127110 date "1978-01-01" @default.
- W2237127110 modified "2023-09-25" @default.
- W2237127110 title "[34] Preparation of nitrogenase" @default.
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- W2237127110 doi "https://doi.org/10.1016/s0076-6879(78)53037-1" @default.
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