Matches in SemOpenAlex for { <https://semopenalex.org/work/W2237213056> ?p ?o ?g. }
- W2237213056 abstract "Lignocellulosic biofuels production is a mechanism that can help reduce our dependence on fossil fuels and enhance the carbon neutrality of transportation fuels. Current biochemical platforms for lignocellulosic biofuel production are not economically viable due to the high costs of hydrolytic enzymes for the conversion of lignocellulose to fermentable sugars. One solution to reduce these costs is incorporation of on-site enzyme production for consolidated bioprocessing of lignocellulose to fermentable sugars. I optimized whole-cell encapsulation parameters that allow for prolonged enzyme production, ease of catalyst separation, and enable incorporation of on-site catalyst production into existing biochemical lignocellulose transformation platforms. Trichoderma reesei RUT-C30 was encapsulated in calcium alginate hydro-gel beads and grown in continuously stirred 250 ml bioreactors containing purified cellulose. Physical and environmental parameters including agitation, reaction volume, and hydrogel diameter and concentration were modulated and compared with unencapsulated controls for cellulase and xylanase activity. The encapsulation matrix retained 90% of the T. reesei biomass. Trichoderma reesei in optimized encapsulation matrices produced similar filter paper units of cellulase activity as unencapsulated controls over a 144-hour period while producing significantly higher xylanase activity than unecapsulated T. reesei" @default.
- W2237213056 created "2016-06-24" @default.
- W2237213056 creator A5028489432 @default.
- W2237213056 date "2012-01-01" @default.
- W2237213056 modified "2023-09-23" @default.
- W2237213056 title "OPTIMIZATION OF A NOVEL TWO-STAGE WHOLE-CELL ENZYME PRODUCTION AND LIGNOCELLULOSE HYDROLYSIS REACTOR DESIGN" @default.
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