FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2237296816> ?p ?o ?g. }

• W2237296816 endingPage "277" @default.
• W2237296816 startingPage "263" @default.
• W2237296816 abstract "Background aims Cell-based therapies have made an impact on the treatment of osteoarthritis; however, the repair and regeneration of thick cartilage defects is an important and growing clinical problem. Next-generation therapies that combine cells with biomaterials may provide improved outcomes. We have developed modular microenvironments that mimic the composition of articular cartilage as a delivery system for consistently differentiated cells. Methods Human bone marrow–derived mesenchymal stem cells (MSC) were embedded in modular microbeads consisting of agarose (AG) supplemented with 0%, 10% and 20% collagen Type II (COL-II) using a water-in-oil emulsion technique. AG and AG/COL-II microbeads were characterized in terms of their structural integrity, size distribution and protein content. The viability of embedded MSC and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages over 3 weeks in culture were also assessed. Results Microbeads made with <20% COL-II were robust, generally spheroidal in shape and 80 ± 10 µm in diameter. MSC viability in microbeads was consistently high over a week in culture, whereas viability in corresponding bulk hydrogels decreased with increasing COL-II content. Osteogenic differentiation of MSC was modestly supported in both AG and AG/COL-II microbeads, whereas adipogenic differentiation was strongly inhibited in COL-II containing microbeads. Chondrogenic differentiation of MSC was clearly promoted in microbeads containing COL-II, compared with pure AG matrices. Conclusions Inclusion of collagen Type II in agarose matrices in microbead format can potentiate chondrogenic differentiation of human MSC. Such compositionally tailored microtissues may find utility for cell delivery in next-generation cartilage repair therapies." @default.
• W2237296816 created "2016-06-24" @default.
• W2237296816 creator A5017163238 @default.
• W2237296816 creator A5035410155 @default.
• W2237296816 creator A5036563674 @default.
• W2237296816 creator A5044948841 @default.
• W2237296816 date "2016-02-01" @default.
• W2237296816 modified "2023-10-15" @default.
• W2237296816 title "Collagen Type II enhances chondrogenic differentiation in agarose-based modular microtissues" @default.
• W2237296816 cites W1603799464 @default.
• W2237296816 cites W1737159960 @default.
• W2237296816 cites W1965425745 @default.
• W2237296816 cites W1967269017 @default.
• W2237296816 cites W1968746947 @default.
• W2237296816 cites W1972896953 @default.
• W2237296816 cites W1975055320 @default.
• W2237296816 cites W1979737063 @default.
• W2237296816 cites W1980567338 @default.
• W2237296816 cites W1981466203 @default.
• W2237296816 cites W1984482077 @default.
• W2237296816 cites W1985073091 @default.
• W2237296816 cites W1988036581 @default.
• W2237296816 cites W1991111225 @default.
• W2237296816 cites W1992895005 @default.
• W2237296816 cites W1995414292 @default.
• W2237296816 cites W1996901223 @default.
• W2237296816 cites W2000228770 @default.
• W2237296816 cites W2003731025 @default.
• W2237296816 cites W2004290723 @default.
• W2237296816 cites W2011038175 @default.
• W2237296816 cites W2011693143 @default.
• W2237296816 cites W2015423555 @default.
• W2237296816 cites W2016363042 @default.
• W2237296816 cites W2017621537 @default.
• W2237296816 cites W2026212186 @default.
• W2237296816 cites W2027246044 @default.
• W2237296816 cites W2031158924 @default.
• W2237296816 cites W2032255522 @default.
• W2237296816 cites W2033399284 @default.
• W2237296816 cites W2038172553 @default.
• W2237296816 cites W2039488563 @default.
• W2237296816 cites W2041401385 @default.
• W2237296816 cites W2047283862 @default.
• W2237296816 cites W2049591756 @default.
• W2237296816 cites W2050273631 @default.
• W2237296816 cites W2051312860 @default.
• W2237296816 cites W2054250836 @default.
• W2237296816 cites W2057991588 @default.
• W2237296816 cites W2060778212 @default.
• W2237296816 cites W2067854519 @default.
• W2237296816 cites W2070396748 @default.
• W2237296816 cites W2074679781 @default.
• W2237296816 cites W2078003030 @default.
• W2237296816 cites W2080112652 @default.
• W2237296816 cites W2081823881 @default.
• W2237296816 cites W2082078691 @default.
• W2237296816 cites W2083544458 @default.
• W2237296816 cites W2086072387 @default.
• W2237296816 cites W2086967343 @default.
• W2237296816 cites W2087578260 @default.
• W2237296816 cites W2090750279 @default.
• W2237296816 cites W2091215928 @default.
• W2237296816 cites W2091298400 @default.
• W2237296816 cites W2091464294 @default.
• W2237296816 cites W2091485881 @default.
• W2237296816 cites W2093458639 @default.
• W2237296816 cites W2100994995 @default.
• W2237296816 cites W2102541192 @default.
• W2237296816 cites W2105812958 @default.
• W2237296816 cites W2121695733 @default.
• W2237296816 cites W2124377144 @default.
• W2237296816 cites W2125578520 @default.
• W2237296816 cites W2129938298 @default.
• W2237296816 cites W2131456542 @default.
• W2237296816 cites W2132610511 @default.
• W2237296816 cites W2133009750 @default.
• W2237296816 cites W2134888071 @default.
• W2237296816 cites W2135763340 @default.
• W2237296816 cites W2151451049 @default.
• W2237296816 cites W2156461747 @default.
• W2237296816 cites W2159387246 @default.
• W2237296816 cites W2159999899 @default.
• W2237296816 cites W2160311975 @default.
• W2237296816 cites W2166856507 @default.
• W2237296816 cites W2169596632 @default.
• W2237296816 cites W2170535560 @default.
• W2237296816 cites W2184277544 @default.
• W2237296816 cites W2196967344 @default.
• W2237296816 cites W2324506750 @default.
• W2237296816 cites W2402191419 @default.
• W2237296816 cites W2613217394 @default.
• W2237296816 cites W2993782230 @default.
• W2237296816 doi "https://doi.org/10.1016/j.jcyt.2015.10.015" @default.
• W2237296816 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4724061" @default.
• W2237296816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26794716" @default.
• W2237296816 hasPublicationYear "2016" @default.
• W2237296816 type Work @default.
• W2237296816 sameAs 2237296816 @default.

• next