FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2237831863> ?p ?o ?g. }

• W2237831863 abstract "Analysis of the primary structure of DNA polymerase e B subunit defined similarities to B subunits of eukaryotic DNA polymerases α, δ and e as well as the small subunits of DNA polymerase DI of Euryarchaeota. Multiple sequence alignment of these proteins revealed the presence of 12 conserved motifs and defined a novel protein superfamily. The members of the B subunit family share a common domain architecture, suggesting a similar fold, and arguing for a conserved function among these proteins. The contribution of human DNA polymerase e to nuclear DNA replication was studied using the antibody K18 that specifically inhibits the activity of this enzyme in vitro. This antibody significantly inhibited DNA synthesis both when microinjected into nuclei of exponentially growing human fibroblasts and in isolated HeLa cell nuclei, but did not inhibit SV40 DNA replication in vitro. These results suggest that the human DNA polymerase e contributes substantially to the replicative synthesis of DNA and emphasises the differences between cellular replication and viral model systems. The human DNA polymerases e and δ were found capable of gap-filling DNA synthesis during nucleotide excision repair in vitro. Both enzymes required PCNA and the clamp loader RFC, and in addition, polymerase δ required Fen-1 to prevent excessive displacement synthesis. Nucleotide excision repair of a defined DNA lesion was completely reconstituted utilising largely recombinant proteins, only ligase I and DNA polymerases δ and e provided as highly purified human enzymes. This system was also utilised to study the role of the transcription factor II H during repair. Human non-homologous end joining of model substrates with different DNA end configurations was studied in HeLa cell extracts. This process depended partially on DNA synthesis as an aphidicolin-dependent DNA polymerase was required for the formation of a subset of end joining products. Experiments with neutralising antibodies reveal that DNA polymerase α but not DNA polymerases β or e, may represent this DNA polymerase activity. Our results indicate that DNA synthesis contributes to the stability of DNA ends, and influences both the efficiency and outcome of the end joining event. Furthermore, our results suggest a minor role of PCNA in non-homologous" @default.
• W2237831863 created "2016-06-24" @default.
• W2237831863 creator A5002105868 @default.
• W2237831863 date "2002-01-01" @default.
• W2237831863 modified "2023-09-23" @default.
• W2237831863 title "The role of DNA polymerases, in particular DNA polymerase ε in DNA repair and replication" @default.
• W2237831863 cites W10876090 @default.
• W2237831863 cites W140427365 @default.
• W2237831863 cites W1483340372 @default.
• W2237831863 cites W1484532115 @default.
• W2237831863 cites W1487822085 @default.
• W2237831863 cites W1488283397 @default.
• W2237831863 cites W1488992604 @default.
• W2237831863 cites W1489580511 @default.
• W2237831863 cites W1490937614 @default.
• W2237831863 cites W1491876634 @default.
• W2237831863 cites W1492423656 @default.
• W2237831863 cites W1497390385 @default.
• W2237831863 cites W1509846551 @default.
• W2237831863 cites W1510511218 @default.
• W2237831863 cites W1513040830 @default.
• W2237831863 cites W1515096181 @default.
• W2237831863 cites W1516514485 @default.
• W2237831863 cites W1519389973 @default.
• W2237831863 cites W1521786414 @default.
• W2237831863 cites W1527517879 @default.
• W2237831863 cites W1530235097 @default.
• W2237831863 cites W1531823089 @default.
• W2237831863 cites W1539508941 @default.
• W2237831863 cites W1540214411 @default.
• W2237831863 cites W1545021361 @default.
• W2237831863 cites W1546888157 @default.
• W2237831863 cites W1547330783 @default.
• W2237831863 cites W1547945395 @default.
• W2237831863 cites W1554806521 @default.
• W2237831863 cites W1554851690 @default.
• W2237831863 cites W1555790021 @default.
• W2237831863 cites W1556435247 @default.
• W2237831863 cites W1556603367 @default.
• W2237831863 cites W1558883070 @default.
• W2237831863 cites W1559981481 @default.
• W2237831863 cites W1560486125 @default.
• W2237831863 cites W1569389754 @default.
• W2237831863 cites W1574197136 @default.
• W2237831863 cites W1576964011 @default.
• W2237831863 cites W1578295668 @default.
• W2237831863 cites W1579061153 @default.
• W2237831863 cites W1579620269 @default.
• W2237831863 cites W1580707139 @default.
• W2237831863 cites W1582522203 @default.
• W2237831863 cites W1585006893 @default.
• W2237831863 cites W1585591827 @default.
• W2237831863 cites W1585955983 @default.
• W2237831863 cites W1589132235 @default.
• W2237831863 cites W1590134197 @default.
• W2237831863 cites W1597009892 @default.
• W2237831863 cites W1599133231 @default.
• W2237831863 cites W1600330290 @default.
• W2237831863 cites W1600623344 @default.
• W2237831863 cites W1600772876 @default.
• W2237831863 cites W1605120960 @default.
• W2237831863 cites W1605174965 @default.
• W2237831863 cites W1605349427 @default.
• W2237831863 cites W1605712430 @default.
• W2237831863 cites W1605809999 @default.
• W2237831863 cites W1607537230 @default.
• W2237831863 cites W1608038670 @default.
• W2237831863 cites W1608564947 @default.
• W2237831863 cites W1611881058 @default.
• W2237831863 cites W1625430625 @default.
• W2237831863 cites W1637596780 @default.
• W2237831863 cites W1654738121 @default.
• W2237831863 cites W1660787335 @default.
• W2237831863 cites W1661736764 @default.
• W2237831863 cites W1671970356 @default.
• W2237831863 cites W1739214237 @default.
• W2237831863 cites W1739291674 @default.
• W2237831863 cites W1754682352 @default.
• W2237831863 cites W1774053018 @default.
• W2237831863 cites W1774715425 @default.
• W2237831863 cites W1798230167 @default.
• W2237831863 cites W1804711025 @default.
• W2237831863 cites W1826078535 @default.
• W2237831863 cites W1833739857 @default.
• W2237831863 cites W1845321470 @default.
• W2237831863 cites W1850062430 @default.
• W2237831863 cites W1858390492 @default.
• W2237831863 cites W1861750438 @default.
• W2237831863 cites W1869411311 @default.
• W2237831863 cites W1873598986 @default.
• W2237831863 cites W1900509644 @default.
• W2237831863 cites W1904872562 @default.
• W2237831863 cites W1906365906 @default.
• W2237831863 cites W1907280881 @default.
• W2237831863 cites W1908345703 @default.
• W2237831863 cites W1908705633 @default.
• W2237831863 cites W1919702696 @default.
• W2237831863 cites W1935789375 @default.
• W2237831863 cites W1943069336 @default.
• W2237831863 cites W1948260762 @default.

• next