FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2238679962> ?p ?o ?g. }

• W2238679962 endingPage "138" @default.
• W2238679962 startingPage "136" @default.
• W2238679962 abstract "The t(11;22)(q24;q12) translocation is present in up to 95% of cases of Ewing’s sarcoma and results in the formation of an EWS-FLI1 fusion gene which encodes a chimeric transcription factor. The proximate role of EWS-FLI1 in the pathogenesis of Ewing’s sarcoma is thought to involve the activation of as yet largely unknown target genes. Many alternative forms of EWS-FLI1 exist because of variations in the locations of the EWS and FLI1 genomic breakpoints. The most common form, designated “type 1,” consists of the first seven exons of EWS joined to exons 6–9 of FLI1 and accounts for approximately 60% of cases. The “type 2” EWS-FLI1 fusion also includes FLI1 exon 5 and is present in another 25%. We and others have observed previously that the type 1 fusion is associated with a significantly better prognosis than the other fusion types. Because EWS-FLI1 is an aberrant transcription factor, we investigated whether these differences in clinical behavior may be correlated to functional differences by comparing transactivation by the type 1 EWS-FLI1 with other types in both heterologous cells (HeLa, NIH3T3) and homologous cells (Ewing’s sarcoma cell lines). In a panel of seven Ewing’s sarcoma cell lines, we found transactivation of a transiently transfected FLI1-responsive reporter construct to be significantly lower in cell lines with the type 1 fusion than in cell lines with the type 2 fusion (P = 0.003). Cotransfection of the same reporter construct with each of a series of seven EWS-FLI1 expression constructs (corresponding to the two major fusion types and five less common types) also showed that type 1 EWS-FLI1 was a significantly weaker transactivator than the type 2 product in both HeLa and NIH3T3 cells (P = 0.003, and P = 0.033, respectively). Electromobility shift assays showed equivalent binding of the type 1 and type 2 EWS-FLI1 to the consensus FLI1-responsive binding site, indicating that differences in transactivation were not due simply to differences in DNA binding affinity. The finding that the type 1 EWS-FLI1 fusion, associated with less aggressive clinical behavior, encodes a less active chimeric transcription factor may provide the basis for a molecular explanation of clinical heterogeneity in Ewing’s sarcoma." @default.
• W2238679962 created "2016-06-24" @default.
• W2238679962 creator A5067389037 @default.
• W2238679962 creator A5087340682 @default.
• W2238679962 date "2016-01-01" @default.
• W2238679962 modified "2023-10-16" @default.
• W2238679962 title "Gender Matters in Biological Research and Medical Practice ∗" @default.
• W2238679962 cites W1990275745 @default.
• W2238679962 cites W2027431314 @default.
• W2238679962 cites W2048060485 @default.
• W2238679962 cites W2074347579 @default.
• W2238679962 cites W2074469498 @default.
• W2238679962 cites W2111277708 @default.
• W2238679962 cites W2169958318 @default.
• W2238679962 cites W2239422374 @default.
• W2238679962 cites W2280612236 @default.
• W2238679962 cites W4293084779 @default.
• W2238679962 doi "https://doi.org/10.1016/j.jacc.2015.11.029" @default.
• W2238679962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26791058" @default.
• W2238679962 hasPublicationYear "2016" @default.
• W2238679962 type Work @default.
• W2238679962 sameAs 2238679962 @default.
• W2238679962 citedByCount "51" @default.
• W2238679962 countsByYear W22386799622016 @default.
• W2238679962 countsByYear W22386799622017 @default.
• W2238679962 countsByYear W22386799622018 @default.
• W2238679962 countsByYear W22386799622019 @default.
• W2238679962 countsByYear W22386799622020 @default.
• W2238679962 countsByYear W22386799622021 @default.
• W2238679962 countsByYear W22386799622022 @default.
• W2238679962 countsByYear W22386799622023 @default.
• W2238679962 crossrefType "journal-article" @default.
• W2238679962 hasAuthorship W2238679962A5067389037 @default.
• W2238679962 hasAuthorship W2238679962A5087340682 @default.
• W2238679962 hasBestOaLocation W22386799621 @default.
• W2238679962 hasConcept C127413603 @default.
• W2238679962 hasConcept C177713679 @default.
• W2238679962 hasConcept C2993312423 @default.
• W2238679962 hasConcept C512399662 @default.
• W2238679962 hasConcept C55587333 @default.
• W2238679962 hasConcept C71924100 @default.
• W2238679962 hasConceptScore W2238679962C127413603 @default.
• W2238679962 hasConceptScore W2238679962C177713679 @default.
• W2238679962 hasConceptScore W2238679962C2993312423 @default.
• W2238679962 hasConceptScore W2238679962C512399662 @default.
• W2238679962 hasConceptScore W2238679962C55587333 @default.
• W2238679962 hasConceptScore W2238679962C71924100 @default.
• W2238679962 hasIssue "2" @default.
• W2238679962 hasLocation W22386799621 @default.
• W2238679962 hasLocation W22386799622 @default.
• W2238679962 hasOpenAccess W2238679962 @default.
• W2238679962 hasPrimaryLocation W22386799621 @default.
• W2238679962 hasRelatedWork W1963663344 @default.
• W2238679962 hasRelatedWork W2015802612 @default.
• W2238679962 hasRelatedWork W2056519704 @default.
• W2238679962 hasRelatedWork W2313740012 @default.
• W2238679962 hasRelatedWork W2403894059 @default.
• W2238679962 hasRelatedWork W2407712986 @default.
• W2238679962 hasRelatedWork W2775568464 @default.
• W2238679962 hasRelatedWork W3152398787 @default.
• W2238679962 hasRelatedWork W4252371801 @default.
• W2238679962 hasRelatedWork W2337494183 @default.
• W2238679962 hasVolume "67" @default.
• W2238679962 isParatext "false" @default.
• W2238679962 isRetracted "false" @default.
• W2238679962 magId "2238679962" @default.
• W2238679962 workType "article" @default.