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• W2238690006 abstract "Abstract Coral tissues commonly contain high concentrations of secondary or special metabolites. These chemicals are identified by spectroscopic analysis after extraction and purification procedures. A new alternative to the conventional techniques is the use of Raman spectroscopy, which is a nondestructive method of analysis suitable to in situ studies of biomaterials as well as chemical compounds. This approach has been successfully demonstrated with corals from the Brazilian coast. Diverse ecological roles have been investigated in crude extracts and pure compounds from the indigenous species Phyllogorgia dilatata , Leptogorgia punicea , L. violacea , and Carijoa riisei and the nonindigenous species Chromonephthea braziliensis , Tubastrea coccinea , and T. tagusensis . Compounds with possible roles in these bioactivities have been quickly characterized by Raman macro- and microspectroscopy: a feeding deterrent sterol from C. braziliensis ; carotenoids from L. violacea , C. riisei , P. dilatata , and Tubastrea spp.; and polyenals from Leptogorgia spp. and C. braziliensis and from purple spots in P. dilatata affected by purpling . Most of the chemical constituents could be identified in tissues and sclerites without the need for any previous treatment. Several Raman vibrational modes are used to correlate chemical species to a chemical substance. The polyenal biochromes could be identified by two “fingerprint” vibrational bands. A complementary analysis can be performed by Raman microimaging, which is a sensitive and selective method of investigating the distribution of compounds in both in situ and in vivo samples. The results obtained by the Raman microimaging studies of chemical compounds present in the surface of healthy and necrotic tissues of P. dilatata may be helpful in evaluating different stages and the extent of infection that causes purpling. Competition for space, antifouling defense, and chemical deterrence are the three main roles traditionally attributed to the chemically mediated bioactivity in corals. Raman spectroscopy may become a valuable nondestructive technique for monitoring the accumulation or production of metabolites during a biological interaction. It also allows information about the distribution and concentration as well as molecular information to be obtained in a very short period of time compared to the usual time and solvent-consuming separation procedures." @default.
• W2238690006 created "2016-06-24" @default.
• W2238690006 creator A5036783684 @default.
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• W2238690006 creator A5066954096 @default.
• W2238690006 creator A5069895193 @default.
• W2238690006 creator A5085052595 @default.
• W2238690006 date "2014-01-01" @default.
• W2238690006 modified "2023-09-23" @default.
• W2238690006 title "New Strategies for Identifying Natural Products of Ecological Significance from Corals" @default.
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