FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2238761636> ?p ?o ?g. }

• W2238761636 endingPage "e0146687" @default.
• W2238761636 startingPage "e0146687" @default.
• W2238761636 abstract "Background Ultra deep sequencing is of increasing use not only in research but also in diagnostics. For implementation of ultra deep sequencing assays in clinical laboratories for routine diagnostics, intra- and inter-laboratory testing are of the utmost importance. Methods A multicenter study was conducted to validate an updated assay design for 454 Life Sciences’ GS FLX Titanium system targeting protease/reverse transcriptase (RTP) and env (V3) regions to identify HIV-1 drug-resistance mutations and determine co-receptor use with high sensitivity. The study included 30 HIV-1 subtype B and 6 subtype non-B samples with viral titers (VT) of 3,940–447,400 copies/mL, two dilution series (52,129–1,340 and 25,130–734 copies/mL), and triplicate samples. Amplicons spanning PR codons 10–99, RT codons 1–251 and the entire V3 region were generated using barcoded primers. Analysis was performed using the GS Amplicon Variant Analyzer and geno2pheno for tropism. For comparison, population sequencing was performed using the ViroSeq HIV-1 genotyping system. Results The median sequencing depth across the 11 sites was 1,829 reads per position for RTP (IQR 592–3,488) and 2,410 for V3 (IQR 786–3,695). 10 preselected drug resistant variants were measured across sites and showed high inter-laboratory correlation across all sites with data (P<0.001). The triplicate samples of a plasmid mixture confirmed the high inter-laboratory consistency (mean% ± stdev: 4.6 ±0.5, 4.8 ±0.4, 4.9 ±0.3) and revealed good intra-laboratory consistency (mean% range ± stdev range: 4.2–5.2 ± 0.04–0.65). In the two dilutions series, no variants >20% were missed, variants 2–10% were detected at most sites (even at low VT), and variants 1–2% were detected by some sites. All mutations detected by population sequencing were also detected by UDS. Conclusions This assay design results in an accurate and reproducible approach to analyze HIV-1 mutant spectra, even at variant frequencies well below those routinely detectable by population sequencing." @default.
• W2238761636 created "2016-06-24" @default.
• W2238761636 creator A5000627402 @default.
• W2238761636 creator A5012477824 @default.
• W2238761636 creator A5032274210 @default.
• W2238761636 creator A5037036136 @default.
• W2238761636 creator A5039483250 @default.
• W2238761636 creator A5048949272 @default.
• W2238761636 creator A5048977896 @default.
• W2238761636 creator A5051679989 @default.
• W2238761636 creator A5052490187 @default.
• W2238761636 creator A5053038328 @default.
• W2238761636 creator A5054673819 @default.
• W2238761636 creator A5061771797 @default.
• W2238761636 creator A5064281779 @default.
• W2238761636 creator A5064954848 @default.
• W2238761636 creator A5068496715 @default.
• W2238761636 creator A5080763997 @default.
• W2238761636 creator A5083768052 @default.
• W2238761636 creator A5084837382 @default.
• W2238761636 creator A5091097962 @default.
• W2238761636 date "2016-01-12" @default.
• W2238761636 modified "2023-10-14" @default.
• W2238761636 title "A Follow-Up of the Multicenter Collaborative Study on HIV-1 Drug Resistance and Tropism Testing Using 454 Ultra Deep Pyrosequencing" @default.
• W2238761636 cites W126321114 @default.
• W2238761636 cites W1524252975 @default.
• W2238761636 cites W1880654616 @default.
• W2238761636 cites W1980355960 @default.
• W2238761636 cites W1997844736 @default.
• W2238761636 cites W1999393895 @default.
• W2238761636 cites W2007390495 @default.
• W2238761636 cites W2025255499 @default.
• W2238761636 cites W2037630163 @default.
• W2238761636 cites W2053485968 @default.
• W2238761636 cites W2060824832 @default.
• W2238761636 cites W2063880708 @default.
• W2238761636 cites W2065740427 @default.
• W2238761636 cites W2083599598 @default.
• W2238761636 cites W2105712999 @default.
• W2238761636 cites W2107387586 @default.
• W2238761636 cites W2114903401 @default.
• W2238761636 cites W2115872602 @default.
• W2238761636 cites W2128114769 @default.
• W2238761636 cites W2128327031 @default.
• W2238761636 cites W2133758885 @default.
• W2238761636 cites W2139311359 @default.
• W2238761636 cites W2140849404 @default.
• W2238761636 cites W2148476904 @default.
• W2238761636 cites W2148938052 @default.
• W2238761636 cites W2151265018 @default.
• W2238761636 cites W2156070837 @default.
• W2238761636 cites W2161381063 @default.
• W2238761636 cites W2163576465 @default.
• W2238761636 cites W2165308292 @default.
• W2238761636 cites W2168518701 @default.
• W2238761636 cites W2169790816 @default.
• W2238761636 cites W2312283795 @default.
• W2238761636 doi "https://doi.org/10.1371/journal.pone.0146687" @default.
• W2238761636 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4710461" @default.
• W2238761636 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26756901" @default.
• W2238761636 hasPublicationYear "2016" @default.
• W2238761636 type Work @default.
• W2238761636 sameAs 2238761636 @default.
• W2238761636 citedByCount "14" @default.
• W2238761636 countsByYear W22387616362015 @default.
• W2238761636 countsByYear W22387616362016 @default.
• W2238761636 countsByYear W22387616362017 @default.
• W2238761636 countsByYear W22387616362018 @default.
• W2238761636 countsByYear W22387616362019 @default.
• W2238761636 countsByYear W22387616362020 @default.
• W2238761636 countsByYear W22387616362021 @default.
• W2238761636 countsByYear W22387616362022 @default.
• W2238761636 crossrefType "journal-article" @default.
• W2238761636 hasAuthorship W2238761636A5000627402 @default.
• W2238761636 hasAuthorship W2238761636A5012477824 @default.
• W2238761636 hasAuthorship W2238761636A5032274210 @default.
• W2238761636 hasAuthorship W2238761636A5037036136 @default.
• W2238761636 hasAuthorship W2238761636A5039483250 @default.
• W2238761636 hasAuthorship W2238761636A5048949272 @default.
• W2238761636 hasAuthorship W2238761636A5048977896 @default.
• W2238761636 hasAuthorship W2238761636A5051679989 @default.
• W2238761636 hasAuthorship W2238761636A5052490187 @default.
• W2238761636 hasAuthorship W2238761636A5053038328 @default.
• W2238761636 hasAuthorship W2238761636A5054673819 @default.
• W2238761636 hasAuthorship W2238761636A5061771797 @default.
• W2238761636 hasAuthorship W2238761636A5064281779 @default.
• W2238761636 hasAuthorship W2238761636A5064954848 @default.
• W2238761636 hasAuthorship W2238761636A5068496715 @default.
• W2238761636 hasAuthorship W2238761636A5080763997 @default.
• W2238761636 hasAuthorship W2238761636A5083768052 @default.
• W2238761636 hasAuthorship W2238761636A5084837382 @default.
• W2238761636 hasAuthorship W2238761636A5091097962 @default.
• W2238761636 hasBestOaLocation W22387616361 @default.
• W2238761636 hasConcept C104317684 @default.
• W2238761636 hasConcept C114851261 @default.
• W2238761636 hasConcept C125009961 @default.
• W2238761636 hasConcept C126322002 @default.
• W2238761636 hasConcept C132917006 @default.

• next