Matches in SemOpenAlex for { <https://semopenalex.org/work/W223877234> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W223877234 abstract "The activation of tissue specific genes relies upon the precise orchestration of a number of events that result in the initiation of transcription upon lineage specification. This process is heavily dictated by the chromatin environment both at the promoter and distal sequences, as well as by the availability of transcription factors necessary for activation. A critical role is played at distal regulatory sequences, which often are the first sites to be engaged by key regulatory proteins. This interaction often promotes a chromatin environment that is necessary for the activation of the gene and results in the recruitment of additional sequence specific factors and a direct interaction with the promoter to initiate transcription. Understanding the properties of enhancer elements for tissue specific genes is important for a clear understanding of the mechanisms of activation. A number of studies have shown that enhancers are marked long before the activation of the gene takes place, in some cases as early as the embryonic stem cell stage. A detailed study described an unmethylated window within the enhancer of the Ptcra locus. Further analysis showed the enhancer mark to be regulated by sequence specific binding factors. These studies lacked the appropriate chromatin environment, which we know to be important. Here we use bacterial artificial chromosome containing the Ptcra locus to demonstrate that the enhancer mark persists in a chromatin context but is not regulated in the manner described in a non-native chromatin context. We then expand our studies to global tissue specific gene expression in order to understand more broadly the regulatory properties the define tissue specific genes. Parsing the mechanisms that drive tissue specific gene expression is critical for an understanding of pluripotency and tissue specificity. Here we use deep chromatin RNA-sequencing to accurately quantify the transcriptome of pluripotent stem cells and four primary differentiated cell types - E14.5 cortical neurons, CD4+ CD8+ thymocytes, bone marrow-derived macrophages and hepatocytes, in mouse. We define tissue specific genes with the broadest dynamic range in expression and define the chromatin properties at their promoters. Separating tissue specific genes with the largest dynamic range in expression allowed us to uncover cell type specific differences in the fundamental promoter properties." @default.
- W223877234 created "2016-06-24" @default.
- W223877234 creator A5052769862 @default.
- W223877234 date "2014-01-01" @default.
- W223877234 modified "2023-09-26" @default.
- W223877234 title "Defining the Chromatin Signatures at Regulatory Regions of Tissue Specific Genes" @default.
- W223877234 hasPublicationYear "2014" @default.
- W223877234 type Work @default.
- W223877234 sameAs 223877234 @default.
- W223877234 citedByCount "0" @default.
- W223877234 crossrefType "journal-article" @default.
- W223877234 hasAuthorship W223877234A5052769862 @default.
- W223877234 hasConcept C101762097 @default.
- W223877234 hasConcept C104317684 @default.
- W223877234 hasConcept C111936080 @default.
- W223877234 hasConcept C137338518 @default.
- W223877234 hasConcept C138885662 @default.
- W223877234 hasConcept C150194340 @default.
- W223877234 hasConcept C165864922 @default.
- W223877234 hasConcept C167227067 @default.
- W223877234 hasConcept C179926584 @default.
- W223877234 hasConcept C21784809 @default.
- W223877234 hasConcept C41895202 @default.
- W223877234 hasConcept C54355233 @default.
- W223877234 hasConcept C56262557 @default.
- W223877234 hasConcept C67258246 @default.
- W223877234 hasConcept C70721500 @default.
- W223877234 hasConcept C83640560 @default.
- W223877234 hasConcept C84597430 @default.
- W223877234 hasConcept C86339819 @default.
- W223877234 hasConcept C86803240 @default.
- W223877234 hasConcept C95444343 @default.
- W223877234 hasConceptScore W223877234C101762097 @default.
- W223877234 hasConceptScore W223877234C104317684 @default.
- W223877234 hasConceptScore W223877234C111936080 @default.
- W223877234 hasConceptScore W223877234C137338518 @default.
- W223877234 hasConceptScore W223877234C138885662 @default.
- W223877234 hasConceptScore W223877234C150194340 @default.
- W223877234 hasConceptScore W223877234C165864922 @default.
- W223877234 hasConceptScore W223877234C167227067 @default.
- W223877234 hasConceptScore W223877234C179926584 @default.
- W223877234 hasConceptScore W223877234C21784809 @default.
- W223877234 hasConceptScore W223877234C41895202 @default.
- W223877234 hasConceptScore W223877234C54355233 @default.
- W223877234 hasConceptScore W223877234C56262557 @default.
- W223877234 hasConceptScore W223877234C67258246 @default.
- W223877234 hasConceptScore W223877234C70721500 @default.
- W223877234 hasConceptScore W223877234C83640560 @default.
- W223877234 hasConceptScore W223877234C84597430 @default.
- W223877234 hasConceptScore W223877234C86339819 @default.
- W223877234 hasConceptScore W223877234C86803240 @default.
- W223877234 hasConceptScore W223877234C95444343 @default.
- W223877234 hasLocation W2238772341 @default.
- W223877234 hasOpenAccess W223877234 @default.
- W223877234 hasPrimaryLocation W2238772341 @default.
- W223877234 hasRelatedWork W1484529414 @default.
- W223877234 hasRelatedWork W1487853120 @default.
- W223877234 hasRelatedWork W1972538685 @default.
- W223877234 hasRelatedWork W1999061898 @default.
- W223877234 hasRelatedWork W2021275962 @default.
- W223877234 hasRelatedWork W2029947491 @default.
- W223877234 hasRelatedWork W2036619779 @default.
- W223877234 hasRelatedWork W2062560066 @default.
- W223877234 hasRelatedWork W2152791321 @default.
- W223877234 hasRelatedWork W2168639912 @default.
- W223877234 hasRelatedWork W2170211828 @default.
- W223877234 hasRelatedWork W2225654837 @default.
- W223877234 hasRelatedWork W2318521053 @default.
- W223877234 hasRelatedWork W2766284392 @default.
- W223877234 hasRelatedWork W2798445611 @default.
- W223877234 hasRelatedWork W2944930303 @default.
- W223877234 hasRelatedWork W3030892594 @default.
- W223877234 hasRelatedWork W3031650634 @default.
- W223877234 hasRelatedWork W3106046749 @default.
- W223877234 hasRelatedWork W2177865089 @default.
- W223877234 isParatext "false" @default.
- W223877234 isRetracted "false" @default.
- W223877234 magId "223877234" @default.
- W223877234 workType "article" @default.