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- W2238883961 abstract "Amino acid analysis is a highly accurate method for characterization of the composition of synthetic peptides. Together with mass spectrometry, it gives a reliable control of peptide quality and quantity before conjugation and immunization. Peptides are hydrolyzed, preferably in gas phase, with 6 M HCl at 110 °C for 20–24 h and the resulting amino acids analyzed by ion-exchange chromatography with post-column ninhydrin derivatization. Depending on the hydrolysis conditions, tryptophan is destroyed, and cysteine also, unless derivatized, and the amides, glutamine and asparagine, are deamidated to glutamic acid and aspartic acid, respectively. Three different ways of calculating results are suggested, and taking the above limitations into account, a quantitation better than 5 % can usually be obtained." @default.
- W2238883961 created "2016-06-24" @default.
- W2238883961 creator A5030190144 @default.
- W2238883961 date "2015-01-01" @default.
- W2238883961 modified "2023-10-17" @default.
- W2238883961 title "Analysis of Peptides and Conjugates by Amino Acid Analysis" @default.
- W2238883961 cites W1641286554 @default.
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- W2238883961 doi "https://doi.org/10.1007/978-1-4939-2999-3_8" @default.
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