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- W2238977020 abstract "Folates are small-molecule metabolites maintaining the normal life activities of organisms.The reduced pterins can participate in folate synthesis.We investigated the effects of the NADPH-dependent pterin reductase(PTR1) from Leishmania spp.on plant folate biosynthesis by over-expressing this gene in model plants Arabidopsis thaliana and tobacco(Nicotiana tabacum).The Leishmania PTR1 gene was cloned and transformed into Arabidopsis and tobacco,respectively,under the control of the constitutive promoter,and folates were measured in the transgenic overexpressors.The results showed that the folate content was enhanced significantly in the transformed plants.The content of 5-formyl-tetrahydrofolate in transgenic Arabidopsis plants was substantially increased,and that of 5-methyl-tetrahydrofolate in transgenic tobacco plants was markedly improved.This research indicated that introduction of PTR1 gene into plants could improve folate biosynthesis,which had provided technological bases for better understanding the rule of folate biosynthesis in plants." @default.
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- W2238977020 date "2012-01-01" @default.
- W2238977020 modified "2023-09-28" @default.
- W2238977020 title "Overexpression of pterin reductase gene PTR1 enhances folate biosynthesis in plants." @default.
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