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• W2239735445 abstract "The use of principal component regression, a multivariate calibration method, in the analysis of in vivo fast-scan cyclic voltammetry data allows for separation of overlapping signal contributions, permitting evaluation of the temporal dynamics of multiple neurotransmitters simultaneously. To accomplish this, the technique relies on information about current-concentration relationships across the scan-potential window gained from analysis of training sets. The ability of the constructed models to resolve analytes depends critically on the quality of these data. Recently, the use of standard training sets obtained under conditions other than those of the experimental data collection (e.g., with different electrodes, animals, or equipment) has been reported. This study evaluates the analyte resolution capabilities of models constructed using this approach from both a theoretical and experimental viewpoint. A detailed discussion of the theory of principal component regression is provided to inform this discussion. The findings demonstrate that the use of standard training sets leads to misassignment of the current-concentration relationships across the scan-potential window. This directly results in poor analyte resolution and, consequently, inaccurate quantitation, which may lead to erroneous conclusions being drawn from experimental data. Thus, it is strongly advocated that training sets be obtained under the experimental conditions to allow for accurate data analysis." @default.
• W2239735445 created "2016-06-24" @default.
• W2239735445 creator A5014607698 @default.
• W2239735445 creator A5055708354 @default.
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• W2239735445 date "2016-01-27" @default.
• W2239735445 modified "2023-09-27" @default.
• W2239735445 title "Failure of Standard Training Sets in the Analysis of Fast-Scan Cyclic Voltammetry Data" @default.
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• W2239735445 doi "https://doi.org/10.1021/acschemneuro.5b00302" @default.
• W2239735445 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5136453" @default.
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• W2239735445 hasPublicationYear "2016" @default.
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