Matches in SemOpenAlex for { <https://semopenalex.org/work/W2239881695> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2239881695 endingPage "1038" @default.
- W2239881695 startingPage "1033" @default.
- W2239881695 abstract "We have investigated the Ca2+ dependency of DNA degradation into nucleosome-sized fragments in intact chromaffin-like PC12 cells and PC12 nuclear fractions. In intact cells we were unable to trigger DNA fragmentation by inducing either transient or sustained elevations of cytoplasmic Ca2+ ([Ca2+]i) with the Ca2+ ionophore ionomycin. On the contrary, DNA fragmentation was induced in intact cells by the intracellular Zn2+ chelator NNN'N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN). To characterize further PC12 cell endonuclease activity, we then investigated digestion by purified PC12 cell fractions of exogenously added plasmids. In nuclear fractions two endonuclease activities were identified: an acidic (pH 5.0) endonuclease activity that was fully Ca2+- and Mg(2+)-independent; and a neutral (pH 7.6) endonuclease activity that was Ca(2+)-independent but Mg(2+)-dependent. Both endonuclease activities were inhibited by Zn2+. Nuclear membrane permeabilization greatly enhanced plasmid digestion at pH 7.6, but not at pH 5.0. This suggests that neutral endonuclease was located in a membrane-bound compartment, whereas acidic endonuclease was freely accessible to the substrate even in the presence of an intact nuclear membrane. In intact nuclei, digestion of genomic DNA could not be triggered by increasing the bivalent cation composition of the medium. On the contrary, in hypotonic medium we observed a large spontaneous nucleolytic DNA degradation that was increased by Zn2+ chelation. However, an acidic pH shift was a potent stimulus for DNA fragmentation in isotonic as well as hypotonic medium." @default.
- W2239881695 created "2016-06-24" @default.
- W2239881695 creator A5003933043 @default.
- W2239881695 creator A5017854085 @default.
- W2239881695 creator A5034720125 @default.
- W2239881695 creator A5041811099 @default.
- W2239881695 creator A5060309123 @default.
- W2239881695 date "1995-11-01" @default.
- W2239881695 modified "2023-10-14" @default.
- W2239881695 title "Ionic regulation of endonuclease activity in PC12 cells" @default.
- W2239881695 doi "https://doi.org/10.1042/bj3111033" @default.
- W2239881695 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1136106" @default.
- W2239881695 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7487921" @default.
- W2239881695 hasPublicationYear "1995" @default.
- W2239881695 type Work @default.
- W2239881695 sameAs 2239881695 @default.
- W2239881695 citedByCount "14" @default.
- W2239881695 countsByYear W22398816952015 @default.
- W2239881695 countsByYear W22398816952023 @default.
- W2239881695 crossrefType "journal-article" @default.
- W2239881695 hasAuthorship W2239881695A5003933043 @default.
- W2239881695 hasAuthorship W2239881695A5017854085 @default.
- W2239881695 hasAuthorship W2239881695A5034720125 @default.
- W2239881695 hasAuthorship W2239881695A5041811099 @default.
- W2239881695 hasAuthorship W2239881695A5060309123 @default.
- W2239881695 hasBestOaLocation W22398816952 @default.
- W2239881695 hasConcept C12554922 @default.
- W2239881695 hasConcept C153911025 @default.
- W2239881695 hasConcept C185592680 @default.
- W2239881695 hasConcept C2777028655 @default.
- W2239881695 hasConcept C2777271071 @default.
- W2239881695 hasConcept C2779377703 @default.
- W2239881695 hasConcept C3017666073 @default.
- W2239881695 hasConcept C552990157 @default.
- W2239881695 hasConcept C55493867 @default.
- W2239881695 hasConcept C57409179 @default.
- W2239881695 hasConcept C79879829 @default.
- W2239881695 hasConcept C86803240 @default.
- W2239881695 hasConceptScore W2239881695C12554922 @default.
- W2239881695 hasConceptScore W2239881695C153911025 @default.
- W2239881695 hasConceptScore W2239881695C185592680 @default.
- W2239881695 hasConceptScore W2239881695C2777028655 @default.
- W2239881695 hasConceptScore W2239881695C2777271071 @default.
- W2239881695 hasConceptScore W2239881695C2779377703 @default.
- W2239881695 hasConceptScore W2239881695C3017666073 @default.
- W2239881695 hasConceptScore W2239881695C552990157 @default.
- W2239881695 hasConceptScore W2239881695C55493867 @default.
- W2239881695 hasConceptScore W2239881695C57409179 @default.
- W2239881695 hasConceptScore W2239881695C79879829 @default.
- W2239881695 hasConceptScore W2239881695C86803240 @default.
- W2239881695 hasIssue "3" @default.
- W2239881695 hasLocation W22398816951 @default.
- W2239881695 hasLocation W22398816952 @default.
- W2239881695 hasLocation W22398816953 @default.
- W2239881695 hasLocation W22398816954 @default.
- W2239881695 hasOpenAccess W2239881695 @default.
- W2239881695 hasPrimaryLocation W22398816951 @default.
- W2239881695 hasRelatedWork W1875856563 @default.
- W2239881695 hasRelatedWork W1986115296 @default.
- W2239881695 hasRelatedWork W2010958611 @default.
- W2239881695 hasRelatedWork W2017008316 @default.
- W2239881695 hasRelatedWork W2023566059 @default.
- W2239881695 hasRelatedWork W2043265428 @default.
- W2239881695 hasRelatedWork W2050812646 @default.
- W2239881695 hasRelatedWork W2140762360 @default.
- W2239881695 hasRelatedWork W2146797217 @default.
- W2239881695 hasRelatedWork W4289255772 @default.
- W2239881695 hasVolume "311" @default.
- W2239881695 isParatext "false" @default.
- W2239881695 isRetracted "false" @default.
- W2239881695 magId "2239881695" @default.
- W2239881695 workType "article" @default.