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- W2240231431 endingPage "25" @default.
- W2240231431 startingPage "1" @default.
- W2240231431 abstract "In the present there is a great necessity of suitable biocatalysts with high process performance, as a “greener” complementary alternative to the chemical synthesis. It is expected that in the coming decade, up to 40% of bulk chemical synthesis processes could be substituted by enzymatic catalysis. The identification and optimization of an appropriate enzyme represent important requirements to obtain a successful and efficient enzymatic process. In this context, the establishment of enzymatic processes in the industry is mainly a problem of finding and optimizing new enzymes. In this sense, nature is the richest reservoir from which enzymes can be isolated because they are continuously changing and evolving as a consequence of natural processes of selection. We are now taking advantages of sequencing and extensive screening technologies to develop enzyme discovery strategiesEnzyme discovery strategies and to identify microbial enzymes with improved and unusual activities and specificities. These approaches, in combination with modern protein engineering methods and distinct combinatorial and rational methods, will increase our chances to generate new stabilized biocatalysts that fit industrial requirements. Here, we review the methodologies, obstacles, and solving problems around metagenomics investigations to screen for enzymes with activities of interest." @default.
- W2240231431 created "2016-06-24" @default.
- W2240231431 creator A5032406789 @default.
- W2240231431 creator A5049865438 @default.
- W2240231431 creator A5051017294 @default.
- W2240231431 creator A5059189176 @default.
- W2240231431 date "2016-01-01" @default.
- W2240231431 modified "2023-10-16" @default.
- W2240231431 title "Metagenomic Mining of Enzyme Diversity" @default.
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