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- W2241134803 abstract "Pear decline (PD) is an economically highly important disease in many pear growing regions. It is associated with a phytoplasma of the 16Sr group X: ‘Candidatus Phytoplasma pyri’. Despite its importance, reliable detection primers for direct identification of the pathogen by PCR are missing. Available PD-specific primers in the 16S-23S rDNA sequence detected only 80% of PDinfected field-collected samples from Southwest Germany. In order to select new primers sequence data of the 16S-23S rDNA region were obtained for two strains of ‘Ca. P. pyri’. PD-specific primers were selected in silico and their specificity was tested with reference strains of the 16Sr group X. They proved to be highly specific for ‘Ca. P. pyri’. The reliability of the primers for PD detection was evaluated with field samples of pear. The detection rate could be improved up to 90% with respect to phytoplasma detection in the same samples with highly sensitive universal ribosomal primers." @default.
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- W2241134803 date "2011-01-01" @default.
- W2241134803 modified "2023-09-26" @default.
- W2241134803 title "Development of specific detection primers for 'Candidatus phytoplasma pyri'" @default.
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