FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2242754256> ?p ?o ?g. }

• W2242754256 endingPage "9528" @default.
• W2242754256 startingPage "9528" @default.
• W2242754256 abstract "9528 Background: Thymidylate synthase (TS), one of the rate-limiting enzymes in the de novo synthesis of DNA, has a unique 28bp tandemly repeated sequence in the promoter region. Patients who are homozygous for the double repeat (2R/2R) of this sequence have been reported to have lower TS expression and better outcome from chemotherapy than patients who are homozygous for the triple repeat (3R/3R). However, loss of heterozygosity (LOH) at the TS locus during tumor development can cause the heterozygous (2R/3R) patients to acquire either the 2R/loss or the loss/3R genotype in their cancer cells, which could affect tumor TS expression as well as clinical outcome. The aim of this study was 1) to verify the relationship between TS mRNA expression and TS genotype in Barrett's associated adenocarcinoma (BA), and 2) to analyze the frequency and timing of LOH at the TS locus in BA and its precursory lesion such as intestinal metaplasia (IM) and high grade dysplasia (HGD). Methods: Sixty-six patients (including 21 with IM, 6 with HGD and 23 with BA) were included in this study. Biopsies were obtained during endoscopy from: (1) normal squamous epithelium at 20cm (HNE); (2) normal squamous epithelium 3cm above the gastroesophageal junction (LNE); (3) IM, HGD or BA. All samples were analyzed for the TS genotype and TS mRNA expression using a quantitative real-time RT-PCR method after laser-capture microdissection. Results: TS mRNA expression from HNE tissue of 3R/3R genotype patients (n=8) was significantly higher than that of 2R/2R genotype patients (1.8-fold; n=14; p=0.0024) and that of 2R/3R genotype patients (1.4-fold; n=44; p=0.0282). Out of 44 patients with heterozygous (2R/3R) genotype in the HNE, no patient lost an allele in the LNE. However, 10 of 18 patients with IM had LOH (55.6%), as had 4/6 patients with HGD (66.7%) and 9/17 patients with BA (52.9%). Conclusions: Our results demonstrate 1) an association between TS genotype and TS expression in normal squamous epithelium and 2) that LOH at the TS locus is a frequent and early event in the IM-BA sequence, and thus tumor and normal genotypes will not be the same in these heterozygous patients. No significant financial relationships to disclose." @default.
• W2242754256 created "2016-06-24" @default.
• W2242754256 creator A5006593239 @default.
• W2242754256 creator A5027836797 @default.
• W2242754256 creator A5029279649 @default.
• W2242754256 creator A5036075728 @default.
• W2242754256 creator A5036527872 @default.
• W2242754256 creator A5057525086 @default.
• W2242754256 creator A5059849760 @default.
• W2242754256 creator A5064011111 @default.
• W2242754256 creator A5068887199 @default.
• W2242754256 creator A5088558286 @default.
• W2242754256 date "2004-07-15" @default.
• W2242754256 modified "2023-09-23" @default.
• W2242754256 title "Thymidylate synthase polymorphism in the development of Barrett's associated adenocarcinoma" @default.
• W2242754256 doi "https://doi.org/10.1200/jco.2004.22.90140.9528" @default.
• W2242754256 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28016832" @default.
• W2242754256 hasPublicationYear "2004" @default.
• W2242754256 type Work @default.
• W2242754256 sameAs 2242754256 @default.
• W2242754256 citedByCount "0" @default.
• W2242754256 crossrefType "journal-article" @default.
• W2242754256 hasAuthorship W2242754256A5006593239 @default.
• W2242754256 hasAuthorship W2242754256A5027836797 @default.
• W2242754256 hasAuthorship W2242754256A5029279649 @default.
• W2242754256 hasAuthorship W2242754256A5036075728 @default.
• W2242754256 hasAuthorship W2242754256A5036527872 @default.
• W2242754256 hasAuthorship W2242754256A5057525086 @default.
• W2242754256 hasAuthorship W2242754256A5059849760 @default.
• W2242754256 hasAuthorship W2242754256A5064011111 @default.
• W2242754256 hasAuthorship W2242754256A5068887199 @default.
• W2242754256 hasAuthorship W2242754256A5088558286 @default.
• W2242754256 hasConcept C104317684 @default.
• W2242754256 hasConcept C11242284 @default.
• W2242754256 hasConcept C121608353 @default.
• W2242754256 hasConcept C123321153 @default.
• W2242754256 hasConcept C126322002 @default.
• W2242754256 hasConcept C135763542 @default.
• W2242754256 hasConcept C142724271 @default.
• W2242754256 hasConcept C143589142 @default.
• W2242754256 hasConcept C153911025 @default.
• W2242754256 hasConcept C180754005 @default.
• W2242754256 hasConcept C2775894508 @default.
• W2242754256 hasConcept C2776497702 @default.
• W2242754256 hasConcept C2780456651 @default.
• W2242754256 hasConcept C2781182431 @default.
• W2242754256 hasConcept C502942594 @default.
• W2242754256 hasConcept C54355233 @default.
• W2242754256 hasConcept C71924100 @default.
• W2242754256 hasConcept C84597430 @default.
• W2242754256 hasConcept C86803240 @default.
• W2242754256 hasConcept C90924648 @default.
• W2242754256 hasConceptScore W2242754256C104317684 @default.
• W2242754256 hasConceptScore W2242754256C11242284 @default.
• W2242754256 hasConceptScore W2242754256C121608353 @default.
• W2242754256 hasConceptScore W2242754256C123321153 @default.
• W2242754256 hasConceptScore W2242754256C126322002 @default.
• W2242754256 hasConceptScore W2242754256C135763542 @default.
• W2242754256 hasConceptScore W2242754256C142724271 @default.
• W2242754256 hasConceptScore W2242754256C143589142 @default.
• W2242754256 hasConceptScore W2242754256C153911025 @default.
• W2242754256 hasConceptScore W2242754256C180754005 @default.
• W2242754256 hasConceptScore W2242754256C2775894508 @default.
• W2242754256 hasConceptScore W2242754256C2776497702 @default.
• W2242754256 hasConceptScore W2242754256C2780456651 @default.
• W2242754256 hasConceptScore W2242754256C2781182431 @default.
• W2242754256 hasConceptScore W2242754256C502942594 @default.
• W2242754256 hasConceptScore W2242754256C54355233 @default.
• W2242754256 hasConceptScore W2242754256C71924100 @default.
• W2242754256 hasConceptScore W2242754256C84597430 @default.
• W2242754256 hasConceptScore W2242754256C86803240 @default.
• W2242754256 hasConceptScore W2242754256C90924648 @default.
• W2242754256 hasIssue "14_suppl" @default.
• W2242754256 hasLocation W22427542561 @default.
• W2242754256 hasOpenAccess W2242754256 @default.
• W2242754256 hasPrimaryLocation W22427542561 @default.
• W2242754256 hasRelatedWork W1974161664 @default.
• W2242754256 hasRelatedWork W1990258492 @default.
• W2242754256 hasRelatedWork W2021720540 @default.
• W2242754256 hasRelatedWork W2052636252 @default.
• W2242754256 hasRelatedWork W2079341249 @default.
• W2242754256 hasRelatedWork W2104882582 @default.
• W2242754256 hasRelatedWork W2241244230 @default.
• W2242754256 hasRelatedWork W3148183718 @default.
• W2242754256 hasRelatedWork W3211546236 @default.
• W2242754256 hasRelatedWork W4220807754 @default.
• W2242754256 hasVolume "22" @default.
• W2242754256 isParatext "false" @default.
• W2242754256 isRetracted "false" @default.
• W2242754256 magId "2242754256" @default.
• W2242754256 workType "article" @default.